The Anorexigenic Action of the Octadecaneuropeptide (ODN) in Goldfish is Mediated Through the MC4R- and Subsequently the CRH Receptor-Signaling Pathways

• Published in: Journal of molecular neuroscience Vol. 42; no. 1; pp. 74 - 79
• Main Authors: Matsuda, Kouhei, Kojima, Kenji, Wada, Kohei, Maruyama, Keisuke, Leprince, Jérôme, Tonon, Marie-Christine, Uchiyama, Minoru, Vaudry, Hubert
• Format: Journal Article
• Language: English
• Published: New York Humana Press Inc 01.09.2010; Springer Nature B.V; Humana Press
• Subjects: alpha -Melanocyte-stimulating hormone; alpha-MSH; alpha-MSH - metabolism; Animals; Appetite Depressants; Appetite Depressants - pharmacology; Benzodiazepines; Biomedical and Life Sciences; Biomedicine; Body weight; Carassius auratus; Cell Behavior; Cell Biology; Cellular Biology; Chemical Sciences; Corticotropin-Releasing Hormone; Corticotropin-Releasing Hormone - genetics; Corticotropin-Releasing Hormone - metabolism; Diazepam Binding Inhibitor; Diazepam Binding Inhibitor - pharmacology; Eating; Eating - drug effects; Feeding Behavior; Feeding Behavior - drug effects; Food; Food consumption; Food intake; Gene expression; Goldfish; Goldfish - physiology; Hypothalamus; Life Sciences; Medicinal Chemistry; Melanocortin; Melanocortin MC4 receptors; Nervous system; Neurobiology; Neurochemistry; Neurology; Neurons and Cognition; Neuropeptides; Neuropeptides - pharmacology; Neurosciences; octadecaneuropeptide; Oligonucleotides; Peptide Fragments; Peptide Fragments - pharmacology; Peptides; Pharmaceutical sciences; Pharmacology; Proopiomelanocortin; Proteins; Proteomics; Receptor, Melanocortin, Type 4; Receptor, Melanocortin, Type 4 - metabolism; Receptors, Corticotropin-Releasing Hormone; Receptors, Corticotropin-Releasing Hormone - metabolism; Signal Transduction; Signal Transduction - drug effects; United States > US; Anorexigenic action; MC4R; Alpha-helical CRH; Octadecaneuropeptide; ICV injection; CRH receptor; HS024; MT II; Goldfish
• ISSN: 0895-8696; 1559-1166
• DOI: 10.1007/s12031-010-9346-9

Intracerebroventricular (ICV) administration of the octadecaneuropeptide (ODN), a peptide derived from diazepam-binding inhibitor, reduces food intake in goldfish as in rodents. However, the neurochemical pathways involved in the anorexigenic action of ODN have not yet been identified in goldfish. Alpha-melanocyte-stimulating hormone (α-MSH), corticotropin-releasing hormone (CRH), and CRH-related peptides play a major role in the control of food consumption in goldfish. In this species, the anorexigenic action of α-MSH is mediated via the CRH/CRH receptor neuronal system. Therefore, in the present study, we examined whether the anorexigenic effect of ODN in goldfish could be mediated through α-MSH and/or CRH neuronal pathways. ICV injection of ODN (10 pmol/g body weight (BW)) significantly reduced food intake, and the anorexigenic effect of ODN was suppressed by ICV preinjection of the melanocortin 4 receptor (MC4R) antagonist HS024 (40 pmol/g BW) or the CRH receptor 1/receptor 2 antagonist α-helical CRH (9–41) (100 pmol/g BW). ICV injection of ODN (10 pmol/g BW) induced a significant increase of proopiomelanocortin mRNA level but had no effect on CRH mRNA level, while ICV injection of the MC4R agonist, melanotan II (100 pmol/g BW), significantly enhanced CRH mRNA expression. These results suggest that, in goldfish, the anorexigenic action of ODN is mediated by the MC4R- and subsequently through the CRH receptor-signaling pathways.