Generation of KCL027 research grade human embryonic stem cell line carrying a mutation in the HTT gene

The KCL027 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (43 trinucleotide repeats; 21 for the normal allele). The ICM was isolated using laser microsurgery and plate...

Full description

Saved in:
Bibliographic Details
Published inStem cell research Vol. 16; no. 2; pp. 274 - 277
Main Authors Jacquet, Laureen, Hewitson, Heema, Wood, Victoria, Kadeva, Neli, Cornwell, Glenda, Codognotto, Stefano, Hobbs, Carl, Stephenson, Emma, Ilic, Dusko
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 01.03.2016
Elsevier
Subjects
Cell Differentiation
Cells, Cultured
Cellular Reprogramming
Comparative Genomic Hybridization
Embryo, Mammalian - cytology
Fertilization in Vitro
Genotype
Histocompatibility Testing
Human Embryonic Stem Cells - cytology
Human Embryonic Stem Cells - metabolism
Humans
Huntingtin Protein - genetics
Karyotype
Lab Resource: Stem Cell Line
Male
Microscopy, Fluorescence
Pedigree
Polymorphism, Genetic
Transcription Factors - genetics
Transcription Factors - metabolism
Trinucleotide Repeats - genetics
Online AccessGet full text

Cover

More Information
Summary:The KCL027 human embryonic stem cell line was derived from an embryo donated for research that carried an autosomal dominant mutation affecting one allele of the HTT gene encoding huntingtin (43 trinucleotide repeats; 21 for the normal allele). The ICM was isolated using laser microsurgery and plated on γ-irradiated human foreskin fibroblasts. Both the derivation and cell line propagation were performed in an animal product-free environment. Pluripotent state and differentiation potential were confirmed by in vitro and in vivo assays.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1873-5061
1876-7753
DOI:10.1016/j.scr.2016.01.008