Antimicrobial peptides are among the antagonistic metabolites produced by Bifidobacterium against Helicobacter pylori
Sixty acid-resistant Bifidobacterium isolates were recovered from human faeces and identified by genus-specific PCR and RAPD-PCR. Helicobacter pylori strains were isolated from gastric biopsies and identified by species-specific PCR. Twenty-four of the 60 Bifidobacterium isolates were considered to...
Saved in:
Published in | International journal of antimicrobial agents Vol. 25; no. 5; pp. 385 - 391 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Elsevier B.V
01.05.2005
Amsterdam Elsevier New York, NY |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Sixty acid-resistant
Bifidobacterium isolates were recovered from human faeces and identified by genus-specific PCR and RAPD-PCR.
Helicobacter pylori strains were isolated from gastric biopsies and identified by species-specific PCR. Twenty-four of the 60
Bifidobacterium isolates were considered to be different strains by RAPD-PCR. Six of the twenty-four different strains were shown to inhibit
H. pylori. These antagonistic effects were related to heat-stable proteinaceous compounds, resistant to heating at 100
°C for 10
min, but sensitive to proteases.
H. pylori stains showed variable resistance to therapeutic antibiotics (metronidazole and clarithromycin), while all the selected bifidobacteria showed intrinsic resistance to metronidazole. These potentially probiotic bifidobacteria were able to inhibit the growth of both antibiotic sensitive and resistant
H. pylori strains. Thus, the synthesis of antimicrobial peptides could be one of the mechanisms of bifidobacteria to combat
H. pylori infections. |
---|---|
Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0924-8579 1872-7913 |
DOI: | 10.1016/j.ijantimicag.2005.01.017 |