Interleukin-11 Inhibits Adipogenesis and Stimulates Myelopoiesis in Human Long-Term Marrow Cultures

• Published in: Blood Vol. 82; no. 5; pp. 1428 - 1435
• Main Authors: Keller, D.C., Du, X.X., Srour, E.F., Hoffman, R., Williams, D.A.
• Format: Journal Article
• Language: English
• Published: Washington, DC Elsevier Inc 01.09.1993; The Americain Society of Hematology
• Subjects: Antigens, CD - analysis; Antigens, CD34; Biological and medical sciences; Bone Marrow - drug effects; Bone Marrow Cells; Cell Adhesion - drug effects; Cell Compartmentation - drug effects; Cell differentiation, maturation, development, hematopoiesis; Cell Division; Cell physiology; Cells, Cultured - drug effects; Fundamental and applied biological sciences. Psychology; HLA-DR Antigens - analysis; Humans; Interleukin-11 - pharmacology; Molecular and cellular biology; Stem Cells - cytology; Stem Cells - immunology; Human; Adipocyte; Treatment; Hematopoiesis; Cytokine; Tissue culture; Interleukin 11; Bone marrow; Myelopoiesis; Cell differentiation
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V82.5.1428.1428

Interleukin-11 (IL-11) is a bone marrow (BM) stromal-derived growth factor that has been shown to stimulate murine myeloid and lymphoid cells both in vitro and in vivo and to inhibit adipogenesis in a murine fibroblast cell line. We have studied the effects of IL-11 on highly purified human BM stem and progenitor cells and on human long-term marrow cultures (LTMC). Adipocyte differentiation is an integral component of murine and human LTMC. IL-11 stimulates myeloid growth as a single cytokine when added to highly enriched CD34+, HLA-DR+ bone marrow cells. IL-11 stimulated no growth in the more primitive CD34+, HLA-DR− population even in the presence of additional cytokines. IL-11 addition to human LTMC resulted in the expansion of myeloid and mixed, but not erythroid, progenitor populations. IL-11 dramatically increased the adherent cell populations, including both stromal cells and macrophages. Treated cultures also showed marked inhibition of fat accumulation in the adherent cells due in part to a block in the differentiation of preadipocytes to adipocytes, as shown by RNA analysis using adipocyte-specific markers. These data show that IL-11 stimulates a more differentiated, although multipotential, progenitor cell in human BM and that LTMC provide a useful model for studying the effects of this cytokine in the context of the hematopoietic microenvironment.