Chronic rhinosinusitis with polyps and without polyps is associated with increased expression of suppressors of cytokine signaling 1 and 3

• Published in: Journal of allergy and clinical immunology Vol. 131; no. 3; pp. 772 - 780
• Main Authors: Park, Se Jin, MD, Kim, Tae Hoon, MD, Jun, Young Joon, MD, Lee, Seung Hoon, MD, Ryu, Hyei Yul, MD, Jung, Kwang Jin, MD, Jung, Jong Yoon, MD, Hwang, Gyu Ho, MD, Lee, Sang Hag, MD
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.03.2013; Elsevier; Elsevier Limited
• Subjects: Adolescent; Adult; Allergy and Immunology; Asthma; Biological and medical sciences; Cell culture; Cells, Cultured; chronic rhinosinusitis with nasal polyps; chronic rhinosinusitis without nasal polyps; Cytokines; Cytokines - genetics; Cytokines - metabolism; Disease; Endothelium; Epithelial Cells; Epithelium; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunohistochemistry; Immunopathology; Inflammation; Interleukin 10; Interleukin 13; Interleukin 4; Interleukin 5; Interleukin 6; Male; Medical sciences; Middle Aged; Mucosa; Nasal Mucosa - metabolism; Nasal Polyps - etiology; Nasal Polyps - metabolism; Non tumoral diseases; Otorhinolaryngology. Stomatology; Pathogenesis; Polymerase chain reaction; Polyps; Rheumatoid arthritis; Rhinitis - complications; Rhinitis - metabolism; rhinosinusitis; RNA, Messenger - metabolism; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; Sinus; Sinuses; Sinusitis - complications; Sinusitis - metabolism; SOCS-1 protein; SOCS1; SOCS3; Studies; submucosal gland; Suppressor of Cytokine Signaling 1 Protein; Suppressor of Cytokine Signaling 3 Protein; Suppressor of Cytokine Signaling Proteins - genetics; Suppressor of Cytokine Signaling Proteins - metabolism; Upper respiratory tract, upper alimentary tract, paranasal sinuses, salivary glands: diseases, semeiology; Western blotting; Young Adult; Chronic rhinosinusitis; CRS with nasal polyps; CRS; CRSwNP; SOCS; chronic rhinosinusitis without nasal polyps; CRSsNP; SOCS3; CT; SOCS1; Computed tomography; chronic rhinosinusitis with nasal polyps; Suppressor of cytokine signaling; cytokines; CRS without nasal polyps; Immunopathology; Nose disease; Nasal polyp; Rhinitis; Upper respiratory tract; Cytokine; Suppressor of cytokine signaling 3; Sinusitis; Chronic; Association; Immunology; ENT disease; Paranasal sinus disease; Suppressor of cytokine signalling; Suppressor cytokine signaling 1
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/j.jaci.2012.12.671

Background Chronic rhinosinusitis with nasal polyps (CRSwNP) or without nasal polyps (CRSsNP) is associated with expression of various cytokines. Suppressors of cytokine signaling (SOCS) regulate cytokine activity in a variety of cells, modulating inflammatory responses. Objective We analyzed the expression and distribution pattern of SOCS1 and SOCS3 in CRSwNP and CRSsNP, and their cytokine-driven expression regulation in sinus mucosa. In addition, the expression levels of various cytokines were evaluated in CRSwNP and CRSsNP. Methods The expression levels of SOCS1 and SOCS3 in CRSwNP and CRSsNP and in control samples were assessed by using real-time PCR, Western blot, and immunohistochemistry. Nasal epithelial cell culture was used to elucidate the effect of IL-4, IL-5, IL-6, IL-10, IL-13, IFN-γ, TNF-α, and TGF-β1 on SOCS1 and SOCS3 expression in sinus mucosa. The expression levels of these cytokines were also evaluated in normal and inflammatory sinus mucosa by using real-time PCR and Western blot. Results The expression levels of SOCS1 and SOCS3 were increased in CRS, irrespective of the presence of nasal polyp, and they were distributed in superficial epithelium, submucosal glands, and vascular endothelium in sinus mucosa. SOCS1 was induced by IL-4, IL-13, IFN-γ, and TNF-α, while SOCS3 expression was upregulated by IL-6, IL-13, IFN-γ, and TNF-α. IL-4 and IL-13 levels were increased in CRSwNP, while IL-4, IFN-γ, TNF-α, and TGF-β1 levels were increased in CRSsNP. Conclusion SOCS1 and SOCS3 are increased in CRS, irrespective of nasal polyp presence. This may be a response to elevated levels of various cytokines increasingly expressed in inflammatory sinus mucosa.