Oxidation sensitizes TRPV2 to chemical and heat stimuli, but not mechanical stimulation

• Published in: Biochemistry and biophysics reports Vol. 28; p. 101173
• Main Authors: Oda, Mai, Fujiwara, Yuichiro, Ishizaki, Yasuki, Shibasaki, Koji
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.12.2021; Elsevier
• Subjects: Heat; Mechanical stimulus; Oxidation; TRP channel; TRPV2; Two-electrode voltage clamp method; Two-electrode voltage clamp method; TRP channel; Heat; TRP; TRPV; ROS; TRPV2; Oxidation; Mechanical stimulus; 2-APB; ChT; LPC
• ISSN: 2405-5808; 2405-5808
• DOI: 10.1016/j.bbrep.2021.101173

The transient receptor potential vanilloid 2 (TRPV2) ion channel is activated by a chemical ligand (2-aminoethoxydiphenyl borate; 2-APB), noxious heat and mechanical stimulation. In a heterologous mammalian cell expression system, the oxidant chloramine T (ChT) sensitizes TRPV2 activation in response to 2-APB and heat by oxidation of methionine residues at positions 528 and 607 in rat TRPV2. Here, we used a Xenopus oocyte expression system to determine whether ChT-mediated oxidation can also sensitize TRPV2 to mechanical stimulation. In this system, we confirmed that ChT sensitized TRPV2 activation in response to 2-APB and heat, but we detected no sensitization to mechanical stimulation. This result suggests that the activation mechanism of TRPV2 by a chemical ligand and heat differs from that for mechanical stimulation. Further, we demonstrated that two-electrode voltage clamp recording in the Xenopus oocyte expression system is an excellent format for high throughput analysis of oxidization of redox-sensitive TRP channels.