Shufeng Jiedu Capsules Alleviate Lipopolysaccharide-Induced Acute Lung Inflammatory Injury via Activation of GPR18 by Verbenalin

• Published in: Cellular physiology and biochemistry Vol. 50; no. 2; pp. 629 - 639
• Main Authors: Yuan, Ying, Liao, Qingwu, Xue, Mingming, Shi, Yujing, Rong, Ling, Song, Zhenju, Tong, Zhaoyang, Zheng, Wuhong, Zhu, Qiang, Cui, Xiaolan, Tao, Zhengang
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.10.2018; Cell Physiol Biochem Press GmbH & Co KG
• Subjects: Acute Lung Injury - chemically induced; Acute Lung Injury - drug therapy; Acute Lung Injury - pathology; Acute respiratory tract infection; Animals; Anti-Inflammatory Agents - chemistry; Anti-Inflammatory Agents - pharmacology; Anti-Inflammatory Agents - therapeutic use; Antibiotics; Apoptosis; Capsules - chemistry; Cyclic AMP-Dependent Protein Kinases - metabolism; Cytokines - analysis; Disease Models, Animal; Drug dosages; Drugs, Chinese Herbal - chemistry; Drugs, Chinese Herbal - pharmacology; Drugs, Chinese Herbal - therapeutic use; Enzymes; FDA approval; Female; GPR18; Infections; Inflammation; Inflammation - pathology; Iridoid Glycosides - chemistry; Iridoid Glycosides - pharmacology; Iridoid Glycosides - therapeutic use; Lipopolysaccharides - toxicity; Lung - drug effects; Lung - metabolism; Lung - pathology; Lungs; Mass spectrometry; Mice; Mice, Inbred C57BL; Mice, Knockout; Original Paper; Peritoneal macrophages; Pulmonary arteries; Receptors, G-Protein-Coupled - agonists; Receptors, G-Protein-Coupled - antagonists & inhibitors; Receptors, G-Protein-Coupled - genetics; Receptors, G-Protein-Coupled - metabolism; RNA Interference; RNA, Small Interfering - metabolism; Scientific imaging; Shufeng Jiedu Capsule; Signal Transduction - drug effects; Tumor necrosis factor-TNF; Verbenalin; China; Shufeng Jiedu Capsule; Verbenalin; Acute respiratory tract infection; GPR18; Peritoneal macrophages
• ISSN: 1015-8987; 1421-9778
• DOI: 10.1159/000494184

Background/Aims: Acute respiratory tract infection (ARTI) is the most common reason for outpatient physician office visits. Although powerful and significant in the treatment of infections, antibiotics used for ARTI inappropriately have been an important contributor to antibiotic resistance. We previously reported that Shufeng Jiedu Capsule (SJC) can effectively amplify anti-inflammatory signaling during infection. In this study, we aimed to systematically explore its composition and the mechanism of its effects in ARTI. Methods: Pseudomonas aeruginosa (PAK) strain was used to generate a mouse model of ARTI, which were then treated with different drugs or compounds to determine the corresponding anti-inflammatory roles. High-performance liquid chromatography-quadrupole time of flight-tandem mass spectrometry. was conducted to detect the chemical compounds in SJC. RNAs from the lung tissues of mice were prepared for microarray analysis to reveal globally altered genes and the pathways involved after SJC treatment. Results: SJC significantly inhibited the expression and secretion of inflammatory factors from PAK-induced mouse lung tissues or lipopolysaccharide-induced peritoneal macrophages. Verbenalin, one of the bioactive compounds identified in SJC, also showed notable anti-inflammatory effects. Microarray data revealed numerous differentially expressed genes among the different treatment groups; here, we focused on studying the role of GPR18. We found that the anti-inflammatory role of verbenalin was attenuated in GPR18 knockout mice compared with wild-type mice, although no statistically significant difference was observed in the untreated PAK-induced mice types. Conclusion: Our data not only showed the chemical composition of SJC, but also demonstrated that verbenalin was a significant anti-inflammatory compound, which may function through GPR18.