Identification of new components of the basal pole of Toxoplasma gondii provides novel insights into its molecular organization and functions

• Published in: Frontiers in cellular and infection microbiology Vol. 12; p. 1010038
• Main Authors: Roumégous, Chloé, Abou Hammoud, Aya, Fuster, Damien, Dupuy, Jean-William, Blancard, Corinne, Salin, Bénédicte, Robinson, Derrick R., Renesto, Patricia, Tardieux, Isabelle, Frénal, Karine
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers 13.10.2022; Frontiers Media S.A
• Subjects: Apicomplexa; basal complex; Cell Division; Cellular and Infection Microbiology; constriction; cytoskeleton; Cytoskeleton - metabolism; Humans; Life Sciences; Microbiology and Parasitology; myosin heavy chain (MHC); Parasitology; Protozoan Proteins - genetics; Protozoan Proteins - metabolism; Toxoplasma - metabolism; Toxoplasma gondii; Toxoplasmosis - parasitology; constriction; Apicomplexa; expansion microscopy; Toxoplasma gondii; cytoskeleton; myosin heavy chain (MHC); basal complex; cell-cell communication; Myosin
• ISSN: 2235-2988; 2235-2988
• DOI: 10.3389/fcimb.2022.1010038

The Toxoplasma gondii tachyzoite is a singled-cell obligate intracellular parasite responsible for the acute phase of toxoplasmosis. This polarized cell exhibits an apical complex, a hallmark of the phylum Apicomplexa, essential for motility, invasion, and egress from the host cell. Located on the opposite end of the cell is the basal complex, an elaborated cytoskeletal structure that also plays critical roles in the lytic cycle of the parasite, being involved in motility, cell division, constriction and cytokinesis, as well as intravacuolar cell-cell communication. Nevertheless, only a few proteins of this structure have been described and functionally assessed. In this study, we used spatial proteomics to identify new basal complex components (BCC), and in situ imaging, including ultrastructure expansion microscopy, to position them. We thus confirmed the localization of nine BCCs out of the 12 selected candidates and assigned them to different sub-compartments of the basal complex, including two new domains located above the basal ring and below the posterior cup. Their functional investigation revealed that none of these BCCs are essential for parasite growth in vitro . However, one BCC is critical for constricting of the basal complex, likely through direct interaction with the class VI myosin heavy chain J (MyoJ), and for gliding motility. Four other BCCs, including a phosphatase and a guanylate-binding protein, are involved in the formation and/or maintenance of the intravacuolar parasite connection, which is required for the rosette organization and synchronicity of cell division.