Effect of Bothrops bilineata snake venom on neutrophil function

• Published in: Toxicon (Oxford) Vol. 76; pp. 143 - 149
• Main Authors: Setubal, Sulamita da Silva, Pontes, Adriana Silva, Nery, Neriane Monteiro, Bastos, Jéssica Silva Félix, Castro, Onassis Boeri, Pires, Weverson Luciano, Zaqueo, Kayena Delaix, Calderon, Leonardo de Azevedo, Stábeli, Rodrigo Guerino, Soares, Andreimar Martins, Zuliani, Juliana Pavan
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 15.12.2013
• Subjects: Animals; Bothrops; Bothrops (Bothriopsis) bilineata venom; Crotalid Venoms - toxicity; Cytokines; Dinoprostone - metabolism; Human; Humans; Hydrogen peroxide; Hydrogen Peroxide - metabolism; In vitro testing; interleukin-6; Interleukin-6 - metabolism; interleukin-8; Interleukin-8 - metabolism; NETs; Neutrophils; Neutrophils - drug effects; Neutrophils - metabolism; Neutrophils - physiology; Reactive oxygen species; Snakes; Toxic; toxicity; Toxicity Tests; Toxicology; venoms; Reactive oxygen species; NETs; Bothrops (Bothriopsis) bilineata venom; Cytokines; Neutrophils
• ISSN: 0041-0101; 1879-3150
• DOI: 10.1016/j.toxicon.2013.09.019

The aim of the study was to evaluate the in vitro effects of Bothrops bilineata crude venom (BbV) on isolated human neutrophil function. We proved that BbV isn't toxic towards human neutrophils. During an incubation of human neutrophils with BbV hydrogen peroxide was produced. Moreover, BbV was able to stimulate neutrophil release of proinflammatory mediators such as IL-8 and IL-6 as well as PGE2 and NETs liberation. There is no data in the literature showing the effect of BbV on the production of IL-6 and IL-8 or NETs liberation by isolated human neutrophils. Taken together our results testify that BbV triggers relevant proinflammatory events in human neutrophils. •B. bilineata venom stimulated neutrophils to release hydrogen peroxide.•B. bilineata venom stimulated neutrophils to release IL-8, IL-6 and PGE2.•B. bilineata venom stimulated neutrophils to release NETs.•The first demonstration of cytokines and NETs liberation from B. bilineata venom.