Complete genome sequence of avian paramyxovirus-3 strain Wisconsin: Evidence for the existence of subgroups within the serotype

• Published in: Virus research Vol. 149; no. 1; pp. 78 - 85
• Main Authors: Kumar, Sachin, Nayak, Baibaswata, Samuel, Arthur S., Xiao, Sa, Collins, Peter L., Samal, Siba K.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.04.2010
• Subjects: 3' Untranslated Regions; 5' Untranslated Regions; Animals; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; APMV; Avian paramyxovirus; Avulavirus - classification; Avulavirus - genetics; Avulavirus - immunology; Cell Line; Chick Embryo; Cluster Analysis; Cross Reactions; Genes, Viral; Genome sequence; Genome, Viral; Genotype; Hemagglutination Inhibition Tests; Human respiratory syncytial virus; Humans; Molecular Sequence Data; Neutralization Tests; Paramyxoviridae; Paramyxovirinae; Paramyxovirus; Phylogeny; RNA, Viral - genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Serotype; Paramyxovirinae; Avian paramyxovirus; Serotype; Genome sequence; APMV; Paramyxoviridae
• ISSN: 0168-1702; 1872-7492
• DOI: 10.1016/j.virusres.2009.12.015

The complete consensus genome sequence was determined for avian paramyxovirus (APMV) serotype 3 strain Wisconsin. The genome is 16,182 nucleotides (nt) in length, consisting of six non-overlapping genes in the order of 3′-N-P/V/W-M-F-HN-L-5′, with a 55-nt leader at its 3′ end and a 681-nt trailer at its 5′ end. Comparison of the APMV-3 strain Wisconsin nt and the aggregate predicted amino acid (aa) sequences with those of APMV-3 strain Netherlands revealed 67% and 78%, identity, respectively. The nt and aa sequence identities between the two APMV-3 strains were lower than between the two antigenic subgroups of human respiratory syncytial virus (81% and 88% identity, respectively) and the two subgroups of human metapeumovirus (80% and 90% identity, respectively). Reciprocal cross-hemagglutination inhibition and cross-neutralization assays using post-infection sera from chickens indicated that strains Wisconsin and Netherlands are highly related antigenically, with only a 2- to 4-fold difference in antibody reactivity between the homologous and heterologous strains. Taken together, our results indicate that the two APMV-3 strains represent a single serotype with two subgroups that differ substantially based on nt and aa sequences, but with only a modest antigenic difference.