Fluorescent labeling of the root cap cells with the bioactive NBD-S chemical probe based on the cellulose biosynthesis inhibition herbicides

• Published in: Biochemistry and biophysics reports Vol. 27; p. 101063
• Main Authors: Tamogami, Shigeru, Agrawal, Ganesh K., Rakwal, Randeep
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2021; Elsevier
• Subjects: Chemical probe; Fluorescence; Indaziflam; NBD; Root cap; Triaziflam; DIEA; Chemical probe; Root cap; DMSO; Triaziflam; Lys; Fluorescence; NBD; Indaziflam; CW; HEPES; NBD-O; NBD-N; Cys; NBD-S; NBD-Cl; CB
• ISSN: 2405-5808; 2405-5808
• DOI: 10.1016/j.bbrep.2021.101063

Development of the methods to examine the molecular targets of biologically active compounds is one of the most important subjects in experimental biology/biochemistry. To evaluate the usability of the (7-nitro-2,1,3-benzoxadiazole)-thioether (NBD-S) probe for this purpose, bioactive chemical probe (1) as the cellulose biosynthesis (CB) inhibitor was synthesized and tested. As a result, a variety of fluorescently-labeled particles and organelles were found in the columella root cap cells of radish plants. Of note, well-defined cellular organelles were clearly recognized in the detaching root cap cells (border-like cells). These results imply that the bioactive NBD-S chemical probe could be a valuable direct-labeling reagent. Analysis of these fluorescent substances would be helpful in providing new information on defined molecular targets and events. [Display omitted] •Nobel S-NBD type chemical probe for cellulose biosynthesis inhibitors was prepared.•This S-NBD type probe was designed for triaziflam and indaziflam.•This S-NBD type probe labeled columella and detaching root cap cells fluorescent.•S-NBD probe would be practical as a target exploring tool compound.