Heritable/conditional genome editing in C. elegans using a CRISPR-Cas9 feeding system
Type II clustered, regularly interspaced, short palindromic repeat (CRISPR)-associated (Cas) system is a novel genome-editing tool for targeted mutagenesis in cultured cells and whole organisms . Recently, the CRISPR-Cas9 system has been applied in C. elegans using various delivery approaches includ...
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Published in | Cell research Vol. 24; no. 7; pp. 886 - 889 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
London
Nature Publishing Group UK
01.07.2014
Nature Publishing Group |
Subjects | |
Online Access | Get full text |
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Summary: | Type II clustered, regularly interspaced, short palindromic repeat (CRISPR)-associated (Cas) system is a novel genome-editing tool for targeted mutagenesis in cultured cells and whole organisms . Recently, the CRISPR-Cas9 system has been applied in C. elegans using various delivery approaches including the transgene method and the direct injection of Cas9 mRNA/single guide RNA (sgRNA) or Cas9-sgRNA ribonucleoproteins . In the present study, we have developed a CRIS- PR-Cas9-based genome-editing tool for C. elegans by delivering gene-specific guide RNA (gRNA) through bacterial feeding to achieve gene disruptions in a time- and labor-saving manner. |
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Bibliography: | Type II clustered, regularly interspaced, short palindromic repeat (CRISPR)-associated (Cas) system is a novel genome-editing tool for targeted mutagenesis in cultured cells and whole organisms . Recently, the CRISPR-Cas9 system has been applied in C. elegans using various delivery approaches including the transgene method and the direct injection of Cas9 mRNA/single guide RNA (sgRNA) or Cas9-sgRNA ribonucleoproteins . In the present study, we have developed a CRIS- PR-Cas9-based genome-editing tool for C. elegans by delivering gene-specific guide RNA (gRNA) through bacterial feeding to achieve gene disruptions in a time- and labor-saving manner. 31-1568/Q These three authors contributed equally to this work. |
ISSN: | 1001-0602 1748-7838 |
DOI: | 10.1038/cr.2014.73 |