Heritable/conditional genome editing in C. elegans using a CRISPR-Cas9 feeding system

Type II clustered, regularly interspaced, short palindromic repeat （CRISPR）-associated （Cas） system is a novel genome-editing tool for targeted mutagenesis in cultured cells and whole organisms . Recently, the CRISPR-Cas9 system has been applied in C. elegans using various delivery approaches includ...

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Bibliographic Details
Published inCell research Vol. 24; no. 7; pp. 886 - 889
Main Authors Liu, Pengpeng, Long, Lijiang, Xiong, Kai, Yu, Bo, Chang, Nannan, Xiong, Jing-Wei, Zhu, Zuoyan, Liu, Dong
Format Journal Article
LanguageEnglish
Published London Nature Publishing Group UK 01.07.2014
Nature Publishing Group
Subjects
631/1647/1511
631/1647/1513/1967/3196
Animals
Animals, Genetically Modified
Base Sequence
Biomedical and Life Sciences
Caenorhabditis elegans - genetics
Cell Biology
Clustered Regularly Interspaced Short Palindromic Repeats
Genetic Engineering - methods
Genome
Letter to the Editor
Life Sciences
Mutagenesis - genetics
培养细胞
基因组
核糖核蛋白
系统
线虫
编辑工具
转基因方法
遗传
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Summary:Type II clustered, regularly interspaced, short palindromic repeat （CRISPR）-associated （Cas） system is a novel genome-editing tool for targeted mutagenesis in cultured cells and whole organisms . Recently, the CRISPR-Cas9 system has been applied in C. elegans using various delivery approaches including the transgene method and the direct injection of Cas9 mRNA/single guide RNA （sgRNA） or Cas9-sgRNA ribonucleoproteins . In the present study, we have developed a CRIS- PR-Cas9-based genome-editing tool for C. elegans by delivering gene-specific guide RNA （gRNA） through bacterial feeding to achieve gene disruptions in a time- and labor-saving manner.
Bibliography:Type II clustered, regularly interspaced, short palindromic repeat （CRISPR）-associated （Cas） system is a novel genome-editing tool for targeted mutagenesis in cultured cells and whole organisms . Recently, the CRISPR-Cas9 system has been applied in C. elegans using various delivery approaches including the transgene method and the direct injection of Cas9 mRNA/single guide RNA （sgRNA） or Cas9-sgRNA ribonucleoproteins . In the present study, we have developed a CRIS- PR-Cas9-based genome-editing tool for C. elegans by delivering gene-specific guide RNA （gRNA） through bacterial feeding to achieve gene disruptions in a time- and labor-saving manner.
31-1568/Q
These three authors contributed equally to this work.
ISSN:1001-0602
1748-7838
DOI:10.1038/cr.2014.73