Effect of individual SCFA on the epithelial barrier of sheep rumen under physiological and acidotic luminal pH conditions

• Published in: Journal of animal science Vol. 96; no. 1; pp. 126 - 142
• Main Authors: Greco, Gabriele, Hagen, Franziska, Meißner, Svenja, Shen, Zanming, Lu, Zhongyan, Amasheh, Salah, Aschenbach, Jörg R
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 01.01.2018
• Subjects: Abundance; Acetic acid; Acetic Acid - pharmacology; Acidosis - physiopathology; Acidosis - veterinary; Animals; Blurring; Butyric Acid - pharmacology; Claudins - genetics; Claudins - metabolism; Conductance; Epithelium - drug effects; Fatty acids; Fatty Acids, Volatile - pharmacology; Female; Fluctuations; Fluorescein; Flux; Gastrointestinal Biology; Hydrogen-Ion Concentration; Immunofluorescence; Localization; mRNA; Mucosa; Permeability - drug effects; pH effects; Physiology; Propionates - pharmacology; Propionic acid; Proteins; Resistance; RNA, Messenger - analysis; Rumen - drug effects; Rumen - metabolism; Sheep; Short circuits; Short-circuit current; Stratum corneum; Tight Junctions - drug effects; Ussing chamber; epithelial barrier; subacute ruminal acidosis; short-chain fatty acids; sheep rumen; tight junction
• ISSN: 0021-8812; 1525-3163
• DOI: 10.1093/jas/skx017

Abstract The objective of this study was to investigate whether individual short-chain fatty acids (SCFA) have a different potential to either regulate the formation of the ruminal epithelial barrier (REB) at physiological pH or to damage the REB at acidotic ruminal pH. Ruminal epithelia of sheep were incubated in Ussing chambers on their mucosal side in buffered solutions (pH 6.1 or 5.1) containing no SCFA (control), 30 mM of either acetate, propionate or butyrate, or 100 mM acetate. Epithelial conductance (Gt), short-circuit current (Isc), and fluorescein flux rates were measured over 7 h. Thereafter, mRNA and protein abundance, as well as localization of the tight junction proteins claudin (Cldn)-1, -4, -7, and occludin were analyzed. At pH 6.1, butyrate increased Gt and decreased Isc, with additional decreases in claudin-7 mRNA and protein abundance (each P < 0.05) and disappearance of Cldn-7 immunosignals from the stratum corneum. By contrast, the mRNA abundance of Cldn-1 and/or Cldn-4 were upregulated by 30 mM propionate, 30 mM butyrate, or 100 mM acetate (P < 0.05), however, without coordinated changes in protein abundance. At luminal pH 5.1, neither Gt, Isc, nor TJ protein abundance was altered in the absence of SCFA; only fluorescein flux rates were slightly increased (P < 0.05) and fluorescein signals were no longer restricted to the stratum corneum. The presence of acetate, propionate, or butyrate at pH 5.1 increased fluorescein flux rates and Gt, and decreased Isc (each P < 0.05). Protein abundance of Cldn-1 was decreased in all SCFA treatments but 30 mM butyrate; abundance of Cldn -4 and -7 was decreased in all SCFA treatments but 30 mM acetate; and abundance of occludin was decreased in all SCFA treatments but 30 mM propionate (each P < 0.05). Immunofluorescence staining of SCFA-treated tissues at pH 5.1 showed disappearance of Cldn-7, discontinuous pattern for Cldn-4 and blurring of occludin and Cldn-1 signals in tight junction complexes. The fluorescein dye appeared to freely diffuse into deeper cell layers. The strongest increase in Gt and consistent decreases in the abundance and immunosignals of tight junction proteins were observed with 100 mM acetate at pH 5.1. We conclude that SCFA may contribute differently to the REB formation at luminal pH 6.1 with possible detrimental effects of butyrate at 30 mM concentration. At luminal pH 5.1, all SCFA elicited REB damage with concentration appearing more critical than SCFA species.