Continuous in vivo Monitoring of Blood Diffusible Calcium Using On-line Microdialysis Sampling Coupled with Flame Atomic Absorption Spectrometry
A direct, rapid and continuous in vivo monitoring of diffusible calcium in the blood of living rabbits has been developed using microdialysis sampling coupled on-line with flame atomic absorption spectrometry. Microdialysates perfused through implanted microdialysis probes were collected with a samp...
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Published in | Analytical Sciences Vol. 21; no. 3; pp. 225 - 229 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Japan
The Japan Society for Analytical Chemistry
01.03.2005
Japan Science and Technology Agency |
Subjects | |
Online Access | Get full text |
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Summary: | A direct, rapid and continuous in vivo monitoring of diffusible calcium in the blood of living rabbits has been developed using microdialysis sampling coupled on-line with flame atomic absorption spectrometry. Microdialysates perfused through implanted microdialysis probes were collected with a sample loop on an injection valve and directly introduced into the flame atomizer by a carrier solution. An ultrapure saline solution (0.9% NaCl, pH 7.2) was used as the perfusion solution at a flow rate of 20 µl min-1 via the microdialysis probe. A 0.1% La solution in 0.5% HNO3 solution was employed as the carrier solution at a nebulizer uptake flow rate of 2.5 ml min-1. The interval for each determination was 2.5 min (2 min of sampling time, 20 s of read time and 10 s of washing time). The performance characteristics of the on-line microdialysis-FAAS system were validated as follows: linearity range, 0 - 100 mg l-1; detection limit (3 σ, n = 7), 3.66 mg l-1; precision (RSD, n = 50), 6.2%. For the evaluation of analytical accuracy, the proposed on-line method was compared with the in vivo no net flux method. The use of an on-line microdialysis-FAAS system permitted the in situ, dynamic and continuous in vivo monitoring of diffusible calcium in the blood of the living rabbits after CaCl2 administration with a temporal resolution of 2.5 min. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0910-6340 1348-2246 |
DOI: | 10.2116/analsci.21.225 |