Immunogenicity of the DNA/Protein Combined Vaccine against COVID-19

• Published in: Bulletin of experimental biology and medicine Vol. 174; no. 2; pp. 246 - 249
• Main Authors: Borgoyakova, M. B., Karpenko, L. I., Merkulyeva, I. A., Shcherbakov, D. N., Rudometov, A. P., Starostina, E. V., Shanshin, D. V., Isaeva, A. A., Nesmeyanova, V. S., Volkova, N. V., Belenkaya, S. V., Volosnikova, E. A., Zadorozhny, A. M., Orlova, L. A., Zaykovskaya, A. V., Pyankov, O. V., Bazhan, S. I., Ilyichev, A. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.12.2022; Springer; Springer Nature B.V
• Subjects: Animals; Antibodies; Antibodies, Viral; Biomedical and Life Sciences; Biomedicine; Cell Biology; Combined vaccines; Comparative analysis; Coronaviruses; COVID-19; COVID-19 - prevention & control; COVID-19 vaccines; COVID-19 Vaccines - genetics; COVID-19 Vaccines - therapeutic use; Deoxyribonucleic acid; DNA; DNA vaccines; Drug therapy, Combination; Gene expression; Health aspects; Humans; Immune response; Immunogenicity; Immunology; Internal Medicine; Laboratory Medicine; Pandemics; Pathology; Plasmids; Proteins; SARS-CoV-2; Severe acute respiratory syndrome coronavirus 2; Spike protein; Vaccines, Combined; Vaccines, DNA - genetics; Virology; subunit vaccines; SARS-CoV-2; DNA vaccines; immune response; DNA/protein vaccines
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-023-05682-9

During the COVID-19 pandemic, the development of prophylactic vaccines, including those based on new platforms, became highly relevant. One such platform is the creation of vaccines combining DNA and protein components in one construct. For the creation of DNA vaccine, we chose the full-length spike protein (S) of the SARS-CoV-2 virus and used the recombinant receptor-binding domain (RBD) of the S protein produced in CHO-K1 cells as a protein component. The immunogenicity of the developed combined vaccine and its individual components was compared and the contribution of each component to the induction of the immune response was analyzed. The combined DNA/protein vaccine possesses the advantages of both underlying approaches and is capable of inducing both humoral (similar to subunit vaccines) and cellular (similar to DNA vaccines) immunity.