Dynamic changes of wound-related miRNAs after application of autologous platelet-rich gel in diabetic wounds

• Published in: Chinese medical journal Vol. 135; no. 21; pp. 2644 - 2646
• Main Authors: Li, Yan, Liang, Yujie, Gao, Yunyi, Chen, Dawei, Ran, Xingwu
• Format: Journal Article
• Language: English
• Published: China Lippincott Williams & Wilkins Ovid Technologies 05.11.2022; Lippincott Williams & Wilkins; Wolters Kluwer
• Subjects: Angiogenesis; Blood Platelets; Collagen; Correspondence; Diabetes; Diabetes Mellitus; Diabetic Foot - therapy; Hogs; Humans; Hyperglycemia; Inflammation; MicroRNAs; MicroRNAs - genetics; Platelet-Rich Plasma; Surgery; Ulcers; Wound healing; Wound Healing - genetics; China
• ISSN: 2542-5641; 0366-6999; 2542-5641
• DOI: 10.1097/CM9.0000000000002358

The study was approved by the Animal Ethics Committee of West China Hospital, Sichuan University (No. [...]the wounds in the two control groups received standard treatment, while the wounds in the diabetic APG group were treated with APG based on standard therapy. Analysis of miRNA relative expression showed that miR-21, miR-146a, and miR-210 (P = 0.046; P = 0.006; P = 0.032) were increased, and miR-29a and miR-155 (P = 0.013; P = 0.001) were decreased during the non-diabetic wound healing process, whereas miR-126 (P = 0.137) showed no statistically significant differences in time factors. miR-29 and miR-146a (P < 0.001; P = 0.013) were increased, and miR-126 and miR-155 (P < 0.001; P = 0.018) were decreased during the diabetic wound healing process, whereas miR-21 and miR-210 (P = 0.164; P = 0.274) showed no statistically significant differences in time factors. [3] miR-21 participates in all stages of wound healing, promoting healing by inhibiting wound inflammation, promoting angiogenesis, increasing collagen deposition, and accelerating re-epithelization. miR-146a and miR-155 are closely associated with the inflammatory response. miR-146a inhibits inflammation by decreasing the expression of pro-inflammatory factors. miR-155 is a proinflammatory factor that can enhance inflammation by activating macrophages. miR-126 and miR-210 are involved in angiogenesis regulation. miR-126 is essential for maintaining the stability and integrity of vascular endothelial cells. miR-210 is a hypoxia-sensitive miRNA that is upregulated in ischemic and hypoxic environments to promote angiogenesis. miR-29 is mainly involved in wound remodeling by inhibiting collagen synthesis, promoting collagen degradation, and reducing matrix protein deposition.