Expression of adhesion and activation molecules in human buccal epithelial cell lines and normal human buccal epithelium in situ

• Published in: Journal of oral pathology & medicine Vol. 30; no. 2; pp. 113 - 120
• Main Authors: Farmer, Ian, Freysdottir, Jona, Dalghous, Abdulbaset M., Fortune, Farida
• Format: Journal Article
• Language: English
• Published: Copenhagen Munksgaard International Publishers 01.02.2001; Blackwell
• Subjects: adhesion molecules; Biological and medical sciences; buccal epithelium; Cadherins - analysis; Carcinoma, Squamous Cell - pathology; CD40 Antigens - analysis; CD58 Antigens - analysis; Cell Adhesion Molecules - analysis; Cell Adhesion Molecules - genetics; Cell Line; Cell Line, Transformed; Cell Transformation, Viral; Culture Media, Serum-Free; Cytokines - pharmacology; Dentistry; Ent. Stomatology; Epithelial Cells - cytology; Epithelial Cells - pathology; fas Receptor - analysis; Flow Cytometry; Gene Expression Regulation; Histocompatibility Antigens Class I - analysis; Humans; Hyaluronan Receptors - analysis; Integrin beta1 - analysis; Intercellular Adhesion Molecule-1 - analysis; Interferon-gamma - pharmacology; Interleukin-4 - pharmacology; Investigative techniques, diagnostic techniques (general aspects); Medical sciences; Mouth Mucosa - cytology; Mouth Mucosa - pathology; Mouth Neoplasms - pathology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; phenotype; Simian virus 40; squamous epithelium; Tumor Cells, Cultured; Up-Regulation; Human; Immunohistochemistry; Flow cytometry; Healthy subject; Intercellular adhesion molecule 1; In situ; Exploration; Activation; Epithelium; Pathology; Molecules; Phenotype; Cell line; Epithelial cell
• ISSN: 0904-2512; 1600-0714
• DOI: 10.1034/j.1600-0714.2001.300208.x

: An in vitro culture system may be used to analyse interactions between T cells and epithelium. We have analysed two human buccal squamous epithelial cell lines: SqCC/Y1, derived from a buccal carcinoma, and SVpgC2a, an SV‐transformed healthy buccal squamous epithelium. Under serum‐free culture conditions, the cell lines resemble normal buccal squamous epithelium in situ in their expression of MHC class I, CD29 (β1 integrin), CD40, CD44, CD54 (ICAM‐1), CD58 (LFA‐3), CD95 (Fas), and E‐cadherin. Furthermore, when the SVpgC2a cell line was treated with T‐cell derived cytokines, upregulation of CD40 expression was observed when the cells were treated with IL‐4, whereas IFN‐γ caused upregulation in expression of CD40, CD54 and MHC class II. These buccal squamous epithelial cell lines, therefore, provide a good tool for analysing interactions between epithelium and T cells in vitro.