Molecular analysis of Staphylococcus aureus isolates associated with staphylococcal food poisoning in South Korea

• Published in: Journal of applied microbiology Vol. 101; no. 4; pp. 864 - 871
• Main Authors: Cha, J.O, Lee, J.K, Jung, Y.H, Yoo, J.I, Park, Y.K, Kim, B.S, Lee, Y.S
• Format: Journal Article
• Language: English
• Published: Oxford, UK Oxford, UK : Blackwell Publishing Ltd 01.10.2006; Blackwell Publishing Ltd; Blackwell Science
• Subjects: Biological and medical sciences; Coagulase - analysis; DNA Fingerprinting; Electrophoresis, Gel, Pulsed-Field; enterotoxin; Enterotoxins - analysis; Food Microbiology; Fundamental and applied biological sciences. Psychology; Genome, Bacterial; Humans; Immune Sera - analysis; Korea - epidemiology; Microbiology; MLST; PFGE; Restriction Mapping; staphylococcal food poisoning; Staphylococcal Food Poisoning - epidemiology; Staphylococcal Food Poisoning - microbiology; Staphylococcus aureus; Staphylococcus aureus - enzymology; Staphylococcus aureus - genetics; Asia; South Korea; Korea; INW, Korea, Rep; Korea, Rep; Toxin; PFGE; MLST; Applied microbiology; Food poisoning; Enterotoxin; Bacteria; Micrococcales; Isolate; Micrococcaceae; staphylococcal food poisoning; Staphylococcus aureus
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1111/j.1365-2672.2006.02957.x

To investigate the molecular epidemiological study of Staphylococcus aureus from staphylococcal food poisoning (SFP) incidents in South Korea. Three hundred and thirty-two strains isolated from ten provinces between June 1999 and January 2002 were characterized by staphylococcal enterotoxin genes, toxic shock syndrome toxin 1 (tst) gene, and exfoliative toxin genes. Toxin genotypes were sea-seh (n = 197), sea (n = 51), sea-seg-sei (n = 14), seg-sei (n = 10), seb (n = 10), seb-sed-seg-sei-sej (n = 3), sea-seg-seh-sei (n = 1), sea-seb (n = 1), sea-sec (n = 1), seg-sei plus eta (n = 4), and sea-seg-sei plus tst (n = 40). Most of the strains could be classified into three clusters of pulsed-field gel electrophoresis (PFGE) types A and B with coagulase type VII and type E with coagulase type IV. Of the ten sequence types (ST), ST1, ST59, and ST30 were frequently showed by multilocus sequence typing. The strain belonging to PFGE pattern A with sea-seh gene, coagulase VII, and ST1 was the most epidemic clone of SFP incidents in Korea.