Cholinesterase activity in pig airways and epithelial cells

• Published in: Fundamental & clinical pharmacology Vol. 11; no. 3; pp. 201 - 205
• Main Authors: Taisne, C., Norel, X., Walch, L., Labat, C., Verriest, C., Mazmanian, GM, Brink, C.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.01.1997; Blackwell
• Subjects: acetylcholinesterase; Acetylcholinesterase - metabolism; airways; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Bronchi - cytology; Bronchi - drug effects; Bronchi - enzymology; butyrylcholinesterase; Butyrylcholinesterase - metabolism; Cells, Cultured; Cyclooxygenase Inhibitors - pharmacology; Enzymes and enzyme inhibitors; Epithelial Cells; epithelium; Epithelium - drug effects; Epithelium - enzymology; Fundamental and applied biological sciences. Psychology; Hydrolases; indomethacin; Indomethacin - pharmacology; Kinetics; Muscle, Smooth - cytology; Muscle, Smooth - drug effects; Muscle, Smooth - enzymology; Swine; Enzyme; Indometacin; Bronchus; Esterases; In vitro; Cholinesterase; Carboxylic ester hydrolases; Pig; Respiratory tract; Vertebrata; Mammalia; Enzymatic activity; Animal; Indoleacetic acid derivatives; Hydrolases; Epithelial cell; Artiodactyla; Ungulata; Biological constant
• ISSN: 0767-3981; 1472-8206
• DOI: 10.1111/j.1472-8206.1997.tb00186.x

Summary— Acetylcholinesterase (AChE, EC 3.1.1.7) and butyrylcholinesterase (BChE, EC 3.1.1.8) activities were detected in bronchial and bronchial epithelial cell homogenates of the pig. In the bronchial homogenates, the maximal upstroke velocity (Vmax) of AChE and the maximal velocity after second substrate fixation (Vss) of BChE were 5.70 ± 0.46 and 7.87 ± 0.81 mU/mg protein, respectively. In the epithelial cell homogenates, a smaller amount of cholinesterase (ChE) was found: Vmax was 0.62 ± 0.29 and Vss was 1.56 ± 0.33 mU/mg protein for AChE and BChE, respectively. AChE activity was increased by 21 ± 5% in the bronchial homogenates and by 54 ± 14% in the epithelial cell homogenates, when intact bronchial segments were incubated with a cyclooxygenase inhibitor, indomethacin (INDO). These results suggest that prostanoids may be involved in the regulation of AChE activity in pig airways.