Cholinesterase activity in pig airways and epithelial cells
Summary— Acetylcholinesterase (AChE, EC 3.1.1.7) and butyrylcholinesterase (BChE, EC 3.1.1.8) activities were detected in bronchial and bronchial epithelial cell homogenates of the pig. In the bronchial homogenates, the maximal upstroke velocity (Vmax) of AChE and the maximal velocity after second s...
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Published in | Fundamental & clinical pharmacology Vol. 11; no. 3; pp. 201 - 205 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford, UK
Blackwell Publishing Ltd
01.01.1997
Blackwell |
Subjects | |
Online Access | Get full text |
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Summary: | Summary— Acetylcholinesterase (AChE, EC 3.1.1.7) and butyrylcholinesterase (BChE, EC 3.1.1.8) activities were detected in bronchial and bronchial epithelial cell homogenates of the pig. In the bronchial homogenates, the maximal upstroke velocity (Vmax) of AChE and the maximal velocity after second substrate fixation (Vss) of BChE were 5.70 ± 0.46 and 7.87 ± 0.81 mU/mg protein, respectively. In the epithelial cell homogenates, a smaller amount of cholinesterase (ChE) was found: Vmax was 0.62 ± 0.29 and Vss was 1.56 ± 0.33 mU/mg protein for AChE and BChE, respectively. AChE activity was increased by 21 ± 5% in the bronchial homogenates and by 54 ± 14% in the epithelial cell homogenates, when intact bronchial segments were incubated with a cyclooxygenase inhibitor, indomethacin (INDO). These results suggest that prostanoids may be involved in the regulation of AChE activity in pig airways. |
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Bibliography: | istex:788440EF3A3635C5E38AF6AB43F8F9C73FD3E1CA ArticleID:FCP186 ark:/67375/WNG-ZL9SWWM3-J ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0767-3981 1472-8206 |
DOI: | 10.1111/j.1472-8206.1997.tb00186.x |