Mitochondrial DNA metabolism in young Psammechinus miliaris embryos
Mitochondrial DNA synthesis was investigated in the early cleavage stages and additionally in the mesenchyme blastula and gastrula stages of the development of P. miliaris. Embryos were incubated for 4 hours in artificial sea water with [ super(3)H] thymidine, after which they were homogenized and t...
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| Published in | FEBS letters Vol. 31; no. 2; pp. 233 - 236 |
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| Main Author | |
| Format | Journal Article |
| Language | English |
| Published |
England
Elsevier B.V
15.04.1973
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| Subjects | |
| Online Access | Get full text |
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| Summary: | Mitochondrial DNA synthesis was investigated in the early cleavage stages and additionally in the mesenchyme blastula and gastrula stages of the development of P. miliaris. Embryos were incubated for 4 hours in artificial sea water with [ super(3)H] thymidine, after which they were homogenized and their mitochondria isolated. Incorporation of [ super(3)H] thymidine into DNA was observed at all stages of development, and the results indicate that mitochondrial DNA metabolism may not begin as a trigger mechanism in a late stage of development, but may in fact be fertilization itself. |
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| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
| ISSN: | 0014-5793 1873-3468 |
| DOI: | 10.1016/0014-5793(73)80111-5 |