Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan

• Published in: Microbiology and immunology Vol. 62; no. 10; p. 673
• Main Authors: Kuramitsu, Madoka, Okuma, Kazu, Nakashima, Makoto, Sato, Tomoo, Sasaki, Daisuke, Hasegawa, Hiroo, Umeki, Kazumi, Kubota, Ryuji, Sasada, Keiko, Sobata, Rieko, Matsumoto, Chieko, Kaneko, Noriaki, Tezuka, Kenta, Matsuoka, Sahoko, Utsunomiya, Atae, Koh, Ki-Ryang, Ogata, Masao, Ishitsuka, Kenji, Taki, Mai, Nosaka, Kisato, Uchimaru, Kaoru, Iwanaga, Masako, Sagara, Yasuko, Yamano, Yoshihisa, Okayama, Akihiko, Miura, Kiyonori, Satake, Masahiro, Saito, Shigeru, Watanabe, Toshiki, Hamaguchi, Isao
• Format: Journal Article
• Language: English
• Published: Australia 01.10.2018
• Subjects: Cell Line, Tumor; Disaccharides - genetics; DNA, Viral - analysis; DNA, Viral - genetics; HTLV-I Infections - genetics; HTLV-I Infections - virology; Human T-lymphotropic virus 1 - genetics; Humans; Japan; Jurkat Cells; Leukemia, T-Cell - virology; Proviruses - genetics; Real-Time Polymerase Chain Reaction - methods; Real-Time Polymerase Chain Reaction - standards; Reference Standards; Viral Load - genetics; standard; human T-cell leukemia virus type 1; quantitative PCR; proviral load

Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were...