Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan

Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were...

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Published inMicrobiology and immunology Vol. 62; no. 10; p. 673
Main Authors Kuramitsu, Madoka, Okuma, Kazu, Nakashima, Makoto, Sato, Tomoo, Sasaki, Daisuke, Hasegawa, Hiroo, Umeki, Kazumi, Kubota, Ryuji, Sasada, Keiko, Sobata, Rieko, Matsumoto, Chieko, Kaneko, Noriaki, Tezuka, Kenta, Matsuoka, Sahoko, Utsunomiya, Atae, Koh, Ki-Ryang, Ogata, Masao, Ishitsuka, Kenji, Taki, Mai, Nosaka, Kisato, Uchimaru, Kaoru, Iwanaga, Masako, Sagara, Yasuko, Yamano, Yoshihisa, Okayama, Akihiko, Miura, Kiyonori, Satake, Masahiro, Saito, Shigeru, Watanabe, Toshiki, Hamaguchi, Isao
Format Journal Article
LanguageEnglish
Published Australia 01.10.2018
Subjects
Cell Line, Tumor
Disaccharides - genetics
DNA, Viral - analysis
DNA, Viral - genetics
HTLV-I Infections - genetics
HTLV-I Infections - virology
Human T-lymphotropic virus 1 - genetics
Humans
Japan
Jurkat Cells
Leukemia, T-Cell - virology
Proviruses - genetics
Real-Time Polymerase Chain Reaction - methods
Real-Time Polymerase Chain Reaction - standards
Reference Standards
Viral Load - genetics
standard
human T-cell leukemia virus type 1
quantitative PCR
proviral load
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Summary:Quantitative PCR (qPCR) of human T-cell leukemia virus type 1 (HTLV-1) provirus is used for HTLV-1 testing and for assessment of risk of HTLV-1-related diseases. In this study, a reference material was developed for standardizing HTLV-1 qPCR. Freeze-dried TL-Om1 cells diluted with Jurkat cells were prepared and an assigned value for proviral load (PVL) of 2.71 copies/100 cells was determined by digital PCR. Nine Japanese laboratories using their own methods evaluated the PVLs of this reference material as 1.08-3.49 copies/100 cells. The maximum difference between laboratories was 3.2-fold. Correcting measured PVLs by using a formula incorporating the assigned value of this reference material should minimize such discrepancies.
ISSN:1348-0421
DOI:10.1111/1348-0421.12644