Determination of neurotransmitter amino acids in mouse central nervous system by CE-LIF

• Published in: Journal of separation science Vol. 36; no. 19; pp. 3264 - 3269
• Main Authors: Xiong, Xu-Jie, Guo, Xiao-Feng, Ge, Xu-Xia, Wang, Hong, Zhang, Hua-Shan
• Format: Journal Article
• Language: English
• Published: Weinheim Blackwell Publishing Ltd 01.10.2013; Wiley; Wiley Subscription Services, Inc
• Subjects: Acetonitrile; Alzheimer's disease; Amino acids; Amino Acids - analysis; Ammonium bromides; Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Buffers; Central nervous system; Central Nervous System - chemistry; Electrophoresis, Capillary - instrumentation; Electrophoresis, Capillary - methods; Fluorescent Dyes - chemistry; Fluorescent probes; Fundamental and applied biological sciences. Psychology; Glutamic acid; Hydrogen-Ion Concentration; MEKC; Mice; Nervous system; Neurotransmitter Agents - analysis; Neurotransmitter amino acids; Neurotransmitters; Non peptidic neurotransmitters, polyamines; Other biological molecules; Running; Spectrometry, Fluorescence - instrumentation; Spectrometry, Fluorescence - methods; Chemical analysis; Fluorescent probes; Alzheimer disease; Micellar electrokinetic capillary chromatography; Central nervous system; Derivatization; Encephalon; Amine borane; MEKC; Degenerative disease; Quantitative analysis; Nervous system diseases; Inhibitory aminoacid; Healthy subject; Rodentia; Neurotransmitter amino acids; Cerebral disorder; Vertebrata; Experimental disease; Mammalia; Analysis method; Mouse; Fluorescent labelling; Animal; Central nervous system disease; Excitatory aminoacid; Neurotransmitter; Fluorescent compound
• ISSN: 1615-9306; 1615-9314; 1615-9314
• DOI: 10.1002/jssc.201300464

An MEKC method with LIF detection has been developed for the determination of seven neurotransmitter amino acids (NAAs) using 1,3,5,7‐tetramethyl‐8‐(N‐hydroxysuccinimidyl butyric ester)difluoroboradiaza‐S‐indacene as the labeling reagent. After derivatization at room temperature for 30 min, the seven target NAAs including glycine, alanine, γ‐aminobutyric acid, taurine, glutamine, glutamic acid, and aspartic acid were separated in running buffer, which consisted of 70 mM pH 4.00 H3PO4/Na3PO4 buffer, 5.5 mM cetyltrimethyl ammonium bromide and 20% v/v acetonitrile within 17 min. The LODs were 2 ∼ 14 × 10−10 M without interference from other coexisting amino acids. The proposed method has been applied to the analysis of NAAs in the central nervous systems of healthy mice and those with Alzheimer's disease with recoveries of 92–104%.