Recombinant non-structural polyprotein 3AB-based serodiagnostic strategy for FMD surveillance in bovines irrespective of vaccination
► Recombinant nonstructural protein 3AB-based indirect ELISA for FMD serosurveillance. ► Diagnostic sensitivity and specificity of 96% and 96.4–99.1%, respectively. ► Detects antibodies from 10 to 900 days postinfection in experimental cattle. ► Higher diagnostic sensitivity than a commercial 3ABC E...
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Published in | Journal of virological methods Vol. 177; no. 2; pp. 184 - 192 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Kidlington
Elsevier B.V
01.11.2011
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | ► Recombinant nonstructural protein 3AB-based indirect ELISA for FMD serosurveillance. ► Diagnostic sensitivity and specificity of 96% and 96.4–99.1%, respectively. ► Detects antibodies from 10 to 900 days postinfection in experimental cattle. ► Higher diagnostic sensitivity than a commercial 3ABC ELISA kit in late phases of infection. ► Occasional postvaccinal 3AB-antibody response warrants more pure vaccines.
In India, the proportion of bovines vaccinated against foot-and-mouth disease (FMD) is increasing since the implementation of the Government supported ‘FMD Control Programme’, and non-structural protein (NSP)-based serological assays for discriminating between antibodies induced by infection or vaccination (DIVA) could be useful. The FMD virus NSP 3AB was expressed in a prokaryotic system and an indirect ELISA (r3AB
3 I-ELISA) was developed and validated as a screening assay for detecting virus in vaccinated bovines. The diagnostic sensitivity of the assay was estimated to be 96%, while the diagnostic specificity varied between the naïve and vaccinates as 99.1% and 96.4%, respectively. This assay could detect antibodies to 3AB (3AB-Ab) from 10 to as late as 900 days post-infection in cattle infected experimentally. The “in-house” assay demonstrated higher sensitivity than a commercial 3ABC ELISA kit particularly with samples obtained from the late stages of infection. Transient post-vaccinal 3AB-Ab response could be detected in one of the three commercial vaccines during the six-month vaccination regimen, which emphasizes the fact that for a DIVA-compatible diagnostic strategy to be a realistic option, all vaccines need to be quality checked for the NSP content. |
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Bibliography: | http://dx.doi.org/10.1016/j.jviromet.2011.08.006 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 |
ISSN: | 0166-0934 1879-0984 |
DOI: | 10.1016/j.jviromet.2011.08.006 |