Recombinant non-structural polyprotein 3AB-based serodiagnostic strategy for FMD surveillance in bovines irrespective of vaccination

• Published in: Journal of virological methods Vol. 177; no. 2; pp. 184 - 192
• Main Authors: Mohapatra, Jajati K., Pandey, Laxmi K., Sanyal, Aniket, Pattnaik, Bramhadev
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.11.2011; Elsevier
• Subjects: Animals; Antibodies; Antibodies, Viral - blood; Antibody Formation; Antigens, Viral - immunology; Biological and medical sciences; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - immunology; Cattle Diseases - virology; diagnostic sensitivity; diagnostic specificity; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - methods; Enzyme-Linked Immunosorbent Assay - standards; Enzyme-Linked Immunosorbent Assay - veterinary; FMD serosurveillance; Foot-and-mouth disease; Foot-and-Mouth Disease - diagnosis; Foot-and-Mouth Disease - immunology; Foot-and-Mouth Disease - virology; Foot-and-mouth disease virus; Foot-and-Mouth Disease Virus - immunology; Foot-and-Mouth Disease Virus - isolation & purification; Foot-and-Mouth Disease Virus - pathogenicity; Fundamental and applied biological sciences. Psychology; Indirect ELISA; Infection; Male; Microbiology; monitoring; polyproteins; Protein Stability; Reagent Kits, Diagnostic - veterinary; Recombinant 3AB; Recombinant Proteins - immunology; screening; Sensitivity and Specificity; Serologic Tests - veterinary; Techniques used in virology; vaccination; Vaccination - veterinary; Vaccines; viral nonstructural proteins; Viral Nonstructural Proteins - immunology; Viral Vaccines - immunology; Virology; viruses; India; Recombinant 3AB; Indirect ELISA; FMD serosurveillance; Bovine; Polyprotein; Vaccination; Method; Vertebrata; Mammalia; Artiodactyla; Veterinary; ELISA assay; Ungulata
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2011.08.006

► Recombinant nonstructural protein 3AB-based indirect ELISA for FMD serosurveillance. ► Diagnostic sensitivity and specificity of 96% and 96.4–99.1%, respectively. ► Detects antibodies from 10 to 900 days postinfection in experimental cattle. ► Higher diagnostic sensitivity than a commercial 3ABC ELISA kit in late phases of infection. ► Occasional postvaccinal 3AB-antibody response warrants more pure vaccines. In India, the proportion of bovines vaccinated against foot-and-mouth disease (FMD) is increasing since the implementation of the Government supported ‘FMD Control Programme’, and non-structural protein (NSP)-based serological assays for discriminating between antibodies induced by infection or vaccination (DIVA) could be useful. The FMD virus NSP 3AB was expressed in a prokaryotic system and an indirect ELISA (r3AB 3 I-ELISA) was developed and validated as a screening assay for detecting virus in vaccinated bovines. The diagnostic sensitivity of the assay was estimated to be 96%, while the diagnostic specificity varied between the naïve and vaccinates as 99.1% and 96.4%, respectively. This assay could detect antibodies to 3AB (3AB-Ab) from 10 to as late as 900 days post-infection in cattle infected experimentally. The “in-house” assay demonstrated higher sensitivity than a commercial 3ABC ELISA kit particularly with samples obtained from the late stages of infection. Transient post-vaccinal 3AB-Ab response could be detected in one of the three commercial vaccines during the six-month vaccination regimen, which emphasizes the fact that for a DIVA-compatible diagnostic strategy to be a realistic option, all vaccines need to be quality checked for the NSP content.