Alginate as an immobilization material for MAb production via encapsulated hybridoma cells

• Published in: Critical reviews in biotechnology Vol. 30; no. 2; pp. 145 - 159
• Main Authors: Selimoglu, S. Mert, Elibol, Murat
• Format: Journal Article
• Language: English
• Published: London Informa UK Ltd 01.06.2010; Taylor & Francis; Informa Healthcare
• Subjects: Alginate; Alginates - chemistry; Alginates - metabolism; Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - isolation & purification; Antibodies, Monoclonal - metabolism; Biological and medical sciences; Biotechnology; Biotechnology - methods; Cells, Immobilized - cytology; Cells, Immobilized - metabolism; Fundamental and applied biological sciences. Psychology; General aspects; Glucuronic Acid - chemistry; Glucuronic Acid - metabolism; Hexuronic Acids - chemistry; Hexuronic Acids - metabolism; Humans; hybridoma; Hybridomas - cytology; Hybridomas - metabolism; immobilization; Immobilization techniques; Methods. Procedures. Technologies; monoclonal antibody production; Encapsulation; Materials; Production; Hybridoma; Immobilization; monoclonal antibody production; Alginate; Alginates; Monoclonal antibody
• ISSN: 0738-8551; 1549-7801
• DOI: 10.3109/07388550903451652

Alginate has been widely used in various applications since its first extraction. What makes this biopolymer useful is its high biocompatibility and humid gelation conditions. Both of these features bring it into prominence as an ideal immobilization material. However, there are some complicated aspects of cell immobilization using alginate biopolymers. This review discuses and clarifies these crucial points, using as an example the bioprocessing of highly fragile cells (hybridoma cells). The review focuses on the cultivation and production of alginate encapsulated cells.