Propolis Inhibits Neurite Outgrowth in Differentiating SH-SY5Y Human Neuroblastoma Cells

• Published in: Toxicological research (Seoul) Vol. 32; no. 3; pp. 239 - 243
• Main Authors: Kim, Han Bit, Yoo, Byung Sun
• Format: Journal Article
• Language: English
• Published: Singapore 한국독성학회 01.07.2016; Springer Singapore; Korean Society of Toxicology
• Subjects: Biomedicine; Original; Original Article; Pharmacology/Toxicology; 예방의학; TGase; SH-SY5Y neuroblastoma cell; Propolis; Neurite outgrowth; GAP-43
• ISSN: 1976-8257; 2234-2753
• DOI: 10.5487/TR.2016.32.3.239

Propolis is a multicomponent, active, complex resinous substance collected by honeybees from a variety of plant sources. We have studied the effect of propolis on neurite outgrowth of SH-SY5Y human neuroblastoma cells induced to differentiate by all-trans-retinoic acid (RA). Propolis, at a concentration of 3 µg/ mL, had no significant effect on the viability of differentiating SH-SY5Y cells. However, the neurite outgrowth of the differentiating SH-SY5Y cells treated with propolis (0.3~3 µg/mL) for 48 hr was significantly inhibited in a dose-dependent manner. Treatment of RA-stimulated differentiating SH-SY5Y cells with 0.3 to 3 µg/mL propolis resulted in decreased level of transglutaminase and 43-kDa growth-associated protein (GAP-43) in a dose-dependent manner. The results indicate that propolis is able to inhibit neurite outgrowth of differentiating SH-SY5Y cells.