Superoxide anion generation response to wound in Arabidopsis hypocotyl cutting

• Published in: Plant signaling & behavior Vol. 16; no. 2; p. 1848086
• Main Authors: Ren, Xufang, Wang, Mengfang, Wang, Yongshun, Huang, Aixia
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 01.02.2021
• Subjects: adventitious root; adventitious roots; Arabidopsis; auxins; behavior; biosynthesis; chlorides; excision; hydrogen peroxide; hypocotyls; mutants; NAD(P)H oxidase (H2O2-forming); Other; reactive oxygen species; rooting; Short Communication; signaling; Superoxide anion; wound; Superoxide anion; wound; signaling; adventitious root; reactive oxygen species
• ISSN: 1559-2316; 1559-2324; 1559-2324
• DOI: 10.1080/15592324.2020.1848086

Cutting is a frequently used model to study the process of adventitious root formation, and excision of cuttings leads to rapid wound response signaling. We recently showed that as a wound signal, reactive oxygen species (ROS, mainly hydrogen peroxide) participate in adventitious root induction of hypocotyl cuttings through regulation of auxin biosynthesis and transport. Here, superoxide anion (O 2 −* ), an early type of ROS, exhibited rapid burst at the cutting site immediately in response to wounding in Arabidopsis hypocotyl cuttings. Diphenylene iodonium chloride (DPI, inhibitor of NADPH oxidase) overwhelmingly suppressed O 2 −* propagation through the hypocotyl. Compared to wild type, O 2 −* burst only occur in cut base, and upward transduction were inhibited completely in NADPH oxidase mutant AtRbohD. These results indicate O 2 −* generation and propagation in response to wound and via NADPH oxidase in adventitious root induction of hypocotyl cuttings.