Engineering and expressing circular RNAs via tRNA splicing

• Published in: RNA biology Vol. 14; no. 8; pp. 978 - 984
• Main Authors: Noto, John J., Schmidt, Casey A., Matera, A. Gregory
• Format: Journal Article
• Language: English
• Published: United States Taylor & Francis 03.08.2017
• Subjects: Animals; Aptamers, Nucleotide - genetics; Aptamers, Nucleotide - metabolism; Archaea; Archaea - genetics; Archaea - metabolism; Back-splicing; biogenesis; circRNA; circular RNA; Drosophila melanogaster - genetics; Drosophila melanogaster - metabolism; ectopic gene expression; Exons; Genetic Engineering - methods; HEK293 Cells; HeLa Cells; Humans; Introns; Mice; Point of Views; RNA - genetics; RNA - metabolism; RNA aptamers; RNA Precursors - genetics; RNA Precursors - metabolism; RNA Splice Sites; RNA Splicing; RNA, Transfer - genetics; RNA, Transfer - metabolism; Spliceosomes - metabolism; Spliceosomes - ultrastructure; transfer RNA; tricRNA; tRNA processing; RNA aptamers; ectopic gene expression; Back-splicing; circRNA; tRNA processing; tricRNA
• ISSN: 1547-6286; 1555-8584; 1555-8584
• DOI: 10.1080/15476286.2017.1317911

Circular (circ)RNAs have recently become a subject of great biologic interest. It is now clear that they represent a diverse and abundant class of RNAs with regulated expression and evolutionarily conserved functions. There are several mechanisms by which RNA circularization can occur in vivo. Here, we focus on the biogenesis of tRNA intronic circular RNAs (tricRNAs) in archaea and animals, and we detail their use as research tools for orthogonal, directed circRNA expression in vivo.