The alignment of MC3T3-E1 osteoblasts on steps of slip traces introduced by dislocation motion

Abstract Bone tissue shows a highly anisotropic microstructure comprising biological apatite and collagen fibrils produced by the mutual activities of bone cells, which dominates its mechanical function. Accordingly, directional control of osteoblasts is crucial for forming anisotropic bone tissue....

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Bibliographic Details
Published inBiomaterials Vol. 33; no. 30; pp. 7327 - 7335
Main Authors Matsugaki, Aira, Aramoto, Gento, Nakano, Takayoshi
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ltd 01.10.2012
Subjects
Advanced Basic Science
Animals
Cell adhesion
Cell Adhesion - drug effects
Cell morphology
Cell Movement - drug effects
Cells, Cultured
Compressive Strength - drug effects
Crystallography
Cytoskeleton - drug effects
Cytoskeleton - metabolism
Dentistry
Mice
Microscopy, Fluorescence
Osteoblast
Osteoblasts - cytology
Osteoblasts - drug effects
Staining and Labeling
Surface Properties
Titanium
Titanium - pharmacology
Osteoblast
Titanium
Crystallography
Cell morphology
Cell adhesion
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Summary:Abstract Bone tissue shows a highly anisotropic microstructure comprising biological apatite and collagen fibrils produced by the mutual activities of bone cells, which dominates its mechanical function. Accordingly, directional control of osteoblasts is crucial for forming anisotropic bone tissue. A new approach was proposed for controlling cell directionality by using crystallographic slip traces caused by dislocation glide. Dislocations were introduced into α-titanium single crystals by plastic deformation of ( 01 1 ¯ 0 ) [ 2 1 ¯ 1 ¯ 0 ] slip system, inducing a step-like structure with acute angles between the surface normal and the slip plane. Topographical properties of step patterning, including step interval and step height, could be controlled by varying the compressive plastic strain. The step geometry introduced by plastic deformation strongly influenced osteoblast elongation, and it aligned preferentially along slip traces. Ti substrates under 10% plastic strain with step height of approximately 300 nm and step interval of 10 μm induced osteoblast alignment most successfully. Actin stress fibers elongated parallel to slip traces, with polarized vinculin accumulation between steps.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2012.06.022