Variant isolates of human metapneumovirus subgroup B genotype 1 in Campinas, Brazil

• Published in: Journal of clinical virology Vol. 42; no. 1; pp. 78 - 81
• Main Authors: da Silva, Luciana Helena Antoniassi, Spilki, Fernando Rosado, Riccetto, Adriana Gut Lopes, de Almeida, Renata Servan, Baracat, Emílio Carlos Elias, Arns, Clarice Weis
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.05.2008; Elsevier Science; Elsevier
• Subjects: Allergy and Immunology; Base Sequence; Biological and medical sciences; Brazil; Cluster Analysis; Female; Fundamental and applied biological sciences. Psychology; Genotype; Genotype B subgroup 1; Hospitals, University; Human metapneumovirus; Human viral diseases; Humans; Infant; Infectious Disease; Infectious diseases; Life Sciences; Male; Medical sciences; Metapneumovirus - classification; Metapneumovirus - genetics; Metapneumovirus - isolation & purification; Microbiology; Miscellaneous; Molecular characterization; Molecular Sequence Data; Nasopharynx - virology; Paramyxoviridae Infections - virology; Paramyxovirus; Phylogeny; Polymorphism, Genetic; RNA, Viral - genetics; Sequence Alignment; Sequence Analysis, DNA; Viral diseases; Virology; South America; Brazil; America; Molecular characterization; Genotype B subgroup 1; Human metapneumovirus; Microbiology; Genotype; Isolate; Virology
• ISSN: 1386-6532; 1873-5967
• DOI: 10.1016/j.jcv.2007.11.010

Abstract Background Human metapneumovirus (HMPV) is a paramyxovirus associated with respiratory illness. The genotypes of HMPV isolates in Brazil have not been well characterized. Objectives To investigate the presence of HMPV in clinical samples collected from pediatric patients of two university hospitals in the region of Campinas (São Paulo, Brazil) and to genotype them by partial sequencing of the HMPV F gene. Study design Nasopharyngeal aspirates were collected from children hospitalized between April and September, 2004 because of acute respiratory infections (ARI). Results We identified HMPV in 8 of 142 (5.6%) clinical samples. We determined through phylogenetic analysis that HMPV isolates in Campinas during the study were clustered within subgroup B genotype 1. Two of the isolates analyzed showed significant differences from previously isolated B1 viruses, when compared to HMPV isolated in South Africa and Canada, and clustered in a separate branch within this genotype. Conclusions In 2004 in our geographic region all HMPV isolates from pediatric patients were in the B1 HMPV genetic group, with two variant isolates.