Extraction of Candida antarctica lipase A from aqueous solutions using imidazolium-based ionic liquids

► The enormous potential of IL-based ABS for lipase extraction is clearly shown. ► First advance to extract a widely used commercial lipase from Candida antarctica. ► Great activity and stability of CaLA in alkylsulfate-based ILs is demonstrated. The suitability of two different imidazolium-based fa...

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Published inSeparation and purification technology Vol. 97; pp. 205 - 210
Main Authors Deive, Francisco J., Rodríguez, Ana, Rebelo, Luís P.N., Marrucho, Isabel M.
Format Journal Article Conference Proceeding
LanguageEnglish
Published Kidlington Elsevier B.V 03.09.2012
Elsevier
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Abstract ► The enormous potential of IL-based ABS for lipase extraction is clearly shown. ► First advance to extract a widely used commercial lipase from Candida antarctica. ► Great activity and stability of CaLA in alkylsulfate-based ILs is demonstrated. The suitability of two different imidazolium-based families of ionic liquids (ILs) involving alkylsulfate and alkylsulfonate anions to act as solvents in an aqueous biphasic system for the extraction of lipase A from Candida antarctica has been investigated. Both high charge–density salts and aminoacids have been studied in order to evaluate both their potential as two-phase forming agents and as stabilizers of the active conformation of the enzyme. Extraction with 1-ethyl-3-methylimidazolium butyl sulfate [C2MIM][C4SO4] with ammonium sulfate affords an enzyme recovery of 99%. The structural conformation of the enzyme was analyzed by Fourier transform infrared data.
AbstractList The suitability of two different imidazolium-based families of ionic liquids (ILs) involving alkylsulfate and alkylsulfonate anions to act as solvents in an aqueous biphasic system for the extraction of lipase A from Candida antarctica has been investigated. Both high charge-density salts and aminoacids have been studied in order to evaluate both their potential as two-phase forming agents and as stabilizers of the active conformation of the enzyme. Extraction with 1-ethyl-3-methylimidazolium butyl sulfate [C2MIM][C4SO4] with ammonium sulfate affords an enzyme recovery of 99%. The structural conformation of the enzyme was analyzed by Fourier transform infrared data.
► The enormous potential of IL-based ABS for lipase extraction is clearly shown. ► First advance to extract a widely used commercial lipase from Candida antarctica. ► Great activity and stability of CaLA in alkylsulfate-based ILs is demonstrated. The suitability of two different imidazolium-based families of ionic liquids (ILs) involving alkylsulfate and alkylsulfonate anions to act as solvents in an aqueous biphasic system for the extraction of lipase A from Candida antarctica has been investigated. Both high charge–density salts and aminoacids have been studied in order to evaluate both their potential as two-phase forming agents and as stabilizers of the active conformation of the enzyme. Extraction with 1-ethyl-3-methylimidazolium butyl sulfate [C2MIM][C4SO4] with ammonium sulfate affords an enzyme recovery of 99%. The structural conformation of the enzyme was analyzed by Fourier transform infrared data.
Author Rebelo, Luís P.N.
Deive, Francisco J.
Rodríguez, Ana
Marrucho, Isabel M.
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  givenname: Isabel M.
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  organization: Instituto de Tecnologia Química e Biológica, www.itqb.unl.pt, UNL, Apartado 127, 2780-157 Oeiras, Portugal
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Keywords Ionic liquids
Aqueous biphasic system
Extraction
Lipase
Green biotechnology
Charge density
Biotechnology
Biphasic system
Aqueous solution
Ionic liquid
Language English
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Snippet ► The enormous potential of IL-based ABS for lipase extraction is clearly shown. ► First advance to extract a widely used commercial lipase from Candida...
The suitability of two different imidazolium-based families of ionic liquids (ILs) involving alkylsulfate and alkylsulfonate anions to act as solvents in an...
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SubjectTerms Applied sciences
Aqueous biphasic system
Candida antarctica
Chemical engineering
Enzymes
Exact sciences and technology
Extraction
Fourier transforms
Green biotechnology
Infrared
Ionic liquids
Lipase
Purification
Title Extraction of Candida antarctica lipase A from aqueous solutions using imidazolium-based ionic liquids
URI https://dx.doi.org/10.1016/j.seppur.2011.12.013
https://search.proquest.com/docview/1069197454
https://search.proquest.com/docview/1654670853
https://search.proquest.com/docview/1800430679
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