Extraction of Candida antarctica lipase A from aqueous solutions using imidazolium-based ionic liquids
► The enormous potential of IL-based ABS for lipase extraction is clearly shown. ► First advance to extract a widely used commercial lipase from Candida antarctica. ► Great activity and stability of CaLA in alkylsulfate-based ILs is demonstrated. The suitability of two different imidazolium-based fa...
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Published in | Separation and purification technology Vol. 97; pp. 205 - 210 |
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Language | English |
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03.09.2012
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Abstract | ► The enormous potential of IL-based ABS for lipase extraction is clearly shown. ► First advance to extract a widely used commercial lipase from Candida antarctica. ► Great activity and stability of CaLA in alkylsulfate-based ILs is demonstrated.
The suitability of two different imidazolium-based families of ionic liquids (ILs) involving alkylsulfate and alkylsulfonate anions to act as solvents in an aqueous biphasic system for the extraction of lipase A from Candida antarctica has been investigated. Both high charge–density salts and aminoacids have been studied in order to evaluate both their potential as two-phase forming agents and as stabilizers of the active conformation of the enzyme. Extraction with 1-ethyl-3-methylimidazolium butyl sulfate [C2MIM][C4SO4] with ammonium sulfate affords an enzyme recovery of 99%. The structural conformation of the enzyme was analyzed by Fourier transform infrared data. |
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AbstractList | The suitability of two different imidazolium-based families of ionic liquids (ILs) involving alkylsulfate and alkylsulfonate anions to act as solvents in an aqueous biphasic system for the extraction of lipase A from Candida antarctica has been investigated. Both high charge-density salts and aminoacids have been studied in order to evaluate both their potential as two-phase forming agents and as stabilizers of the active conformation of the enzyme. Extraction with 1-ethyl-3-methylimidazolium butyl sulfate [C2MIM][C4SO4] with ammonium sulfate affords an enzyme recovery of 99%. The structural conformation of the enzyme was analyzed by Fourier transform infrared data. ► The enormous potential of IL-based ABS for lipase extraction is clearly shown. ► First advance to extract a widely used commercial lipase from Candida antarctica. ► Great activity and stability of CaLA in alkylsulfate-based ILs is demonstrated. The suitability of two different imidazolium-based families of ionic liquids (ILs) involving alkylsulfate and alkylsulfonate anions to act as solvents in an aqueous biphasic system for the extraction of lipase A from Candida antarctica has been investigated. Both high charge–density salts and aminoacids have been studied in order to evaluate both their potential as two-phase forming agents and as stabilizers of the active conformation of the enzyme. Extraction with 1-ethyl-3-methylimidazolium butyl sulfate [C2MIM][C4SO4] with ammonium sulfate affords an enzyme recovery of 99%. The structural conformation of the enzyme was analyzed by Fourier transform infrared data. |
Author | Rebelo, Luís P.N. Deive, Francisco J. Rodríguez, Ana Marrucho, Isabel M. |
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Keywords | Ionic liquids Aqueous biphasic system Extraction Lipase Green biotechnology Charge density Biotechnology Biphasic system Aqueous solution Ionic liquid |
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Snippet | ► The enormous potential of IL-based ABS for lipase extraction is clearly shown. ► First advance to extract a widely used commercial lipase from Candida... The suitability of two different imidazolium-based families of ionic liquids (ILs) involving alkylsulfate and alkylsulfonate anions to act as solvents in an... |
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SubjectTerms | Applied sciences Aqueous biphasic system Candida antarctica Chemical engineering Enzymes Exact sciences and technology Extraction Fourier transforms Green biotechnology Infrared Ionic liquids Lipase Purification |
Title | Extraction of Candida antarctica lipase A from aqueous solutions using imidazolium-based ionic liquids |
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