Extraction of Candida antarctica lipase A from aqueous solutions using imidazolium-based ionic liquids

• Published in: Separation and purification technology Vol. 97; pp. 205 - 210
• Main Authors: Deive, Francisco J., Rodríguez, Ana, Rebelo, Luís P.N., Marrucho, Isabel M.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Kidlington Elsevier B.V 03.09.2012; Elsevier
• Subjects: Applied sciences; Aqueous biphasic system; Candida antarctica; Chemical engineering; Enzymes; Exact sciences and technology; Extraction; Fourier transforms; Green biotechnology; Infrared; Ionic liquids; Lipase; Purification; Ionic liquids; Aqueous biphasic system; Extraction; Lipase; Green biotechnology; Charge density; Biotechnology; Biphasic system; Aqueous solution; Ionic liquid
• ISSN: 1383-5866; 1873-3794
• DOI: 10.1016/j.seppur.2011.12.013

► The enormous potential of IL-based ABS for lipase extraction is clearly shown. ► First advance to extract a widely used commercial lipase from Candida antarctica. ► Great activity and stability of CaLA in alkylsulfate-based ILs is demonstrated. The suitability of two different imidazolium-based families of ionic liquids (ILs) involving alkylsulfate and alkylsulfonate anions to act as solvents in an aqueous biphasic system for the extraction of lipase A from Candida antarctica has been investigated. Both high charge–density salts and aminoacids have been studied in order to evaluate both their potential as two-phase forming agents and as stabilizers of the active conformation of the enzyme. Extraction with 1-ethyl-3-methylimidazolium butyl sulfate [C2MIM][C4SO4] with ammonium sulfate affords an enzyme recovery of 99%. The structural conformation of the enzyme was analyzed by Fourier transform infrared data.