Novel monoclonal antibodies that bind to wild and fixed rabies virus strains

• Published in: Journal of virological methods Vol. 175; no. 1; pp. 66 - 73
• Main Authors: Zanluca, Camila, Aires, Luan Renato dos Passos, Mueller, Paula Pazzini, Santos, Vanessa Valgas dos, Carrieri, Maria Luiza, Pinto, Aguinaldo Roberto, Zanetti, Carlos Roberto
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.07.2011; Elsevier
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Viral - immunology; Antigens, Viral - analysis; Antigens, Viral - immunology; Biological and medical sciences; Brazilian wild rabies isolates; Cricetinae; diagnostic techniques; Enzyme-Linked Immunosorbent Assay; Fixed rabies virus strains; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Glycoproteins; Glycoproteins - immunology; humans; Immunoassays; Immunoblotting; Immunofluorescence; Immunoglobulin G; Immunoglobulin G - immunology; Immunoglobulin M; Immunoglobulin M - immunology; Immunoreactivity; Mice; Mice, Inbred BALB C; Microbiology; Monoclonal antibodies; Monoclonal antibody; neutralization; Neutralization Tests; Rabies; Rabies - diagnosis; Rabies - immunology; Rabies virus; Rabies virus - immunology; Techniques used in virology; Vaccines; Virology; viruses; Monoclonal antibody; Brazilian wild rabies isolates; Fixed rabies virus strains; Rabies; Nervous system diseases; Method; Rabies virus; Strain; Infection; Virus; Mononegavirales; Rhabdoviridae; Viral disease; Lyssavirus; Isolate
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/j.jviromet.2011.04.019

► Ten monoclonal antibodies against rabies virus were produced and characterized. ► MAb (8D11) recognizes rabies virus glycoprotein and neutralizes rabies virus in vitro. ► MAb 6H8 was found to be reactive against 35 Brazilian rabies virus isolates. ► MAbs LIA 02, 6H8, and 9C7 reacted against 18 vaccine strains. Ten monoclonal antibodies (MAbs) against rabies virus, including IgG3κ, IgG2aκ, IgMκ, and an IgG2bκ isotype, were produced and characterized using neutralization, ELISA, immunodot-blot, and immunofluorescence assays. MAb 8D11, which recognized rabies virus glycoprotein, was found to neutralize rabies virus in vitro. When submitted to an immunofluorescence assay, seven MAbs showed different reactivity against 35 Brazilian rabies virus isolates. Three MAbs (LIA 02, 3E6, and 9C7) only failed to recognize one or two virus isolates, whereas MAb 6H8 was found to be reactive against all virus isolates tested. MAbs were also evaluated for their immunoreactivity against fixed rabies virus strains present in human and veterinary commercial vaccines. MAbs LIA 02, 6H8, and 9C7 reacted against all vaccine strains, while the remaining MAbs recognized at least 76% of vaccine strains tested. This research provides a set of MAbs with potential application for improving existing or developing new diagnostic tests and immunoassays.