Radially oriented collagen scaffold with SDF-1 promotes osteochondral repair by facilitating cell homing

• Published in: Biomaterials Vol. 39; pp. 114 - 123
• Main Authors: Chen, Pengfei, Tao, Jiadong, Zhu, Shouan, Cai, Youzhi, Mao, Qijiang, Yu, Dongsheng, Dai, Jun, Ouyang, HongWei
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.01.2015
• Subjects: Advanced Basic Science; Animals; Biocompatibility; Biomedical materials; Cartilage; Cartilage defect repair; Cell Movement - drug effects; Cell Proliferation - drug effects; Cell Survival - drug effects; Chemokine CXCL12 - chemistry; Chemokine CXCL12 - pharmacology; Chondrogenesis - drug effects; Collagen - chemistry; Collagen scaffolds; Collagens; Defects; Dentistry; Immunohistochemistry; Male; Osteochondral tissue engineering; Rabbits; Regeneration; Repair; Scaffolds; SDF-1; Tissue Engineering - methods; Tissue Scaffolds - chemistry; Wound Healing - drug effects; Collagen scaffolds; Osteochondral tissue engineering; SDF-1; Cartilage defect repair
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2014.10.049

Abstract The migration of cells from the side and the bottom of the defect is important in osteochondral defect healing. Here, we designed a novel collagen scaffold that possessed channels in both the horizontal and the vertical directions, along with stromal cell-derived factor-1 (SDF-1) to enhance osteochondral regeneration by facilitating cell homing. Firstly we fabricated the radially oriented and random collagen scaffolds, then tested their properties. The radially oriented collagen scaffold had better mechanical properties than the random scaffold, but both supported cell proliferation well. Then we measured the migration of BMSCs in the scaffolds in vitro . The radially oriented collagen scaffold effectively promoted their migration, and this effect was further facilitated by addition of SDF-1. Moreover, we created osteochondral defects in rabbits, and implanted them with random or oriented collagen scaffolds with or without SDF-1, and evaluated cartilage and subchondral bone regeneration at 6 and 12 weeks after surgery. Cartilage regeneration with both the radially oriented scaffold and SDF-1 effectively promoted repair of the cartilage defect. Our results confirmed that the implantation of the radially oriented channel collagen scaffold with SDF-1 could be a promising strategy for osteochondral repair.