Isolation and identification of cyclic lipopeptides from Paenibacillus ehimensis, strain IB-X-b

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 973; pp. 9 - 16
• Main Authors: Aktuganov, Gleb, Jokela, Jouni, Kivelä, Henri, Khalikova, Elvira, Melentjev, Alexander, Galimzianova, Nailia, Kuzmina, Lyudmila, Kouvonen, Petri, Himanen, Juha-Pekka, Susi, Petri, Korpela, Timo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.12.2014
• Subjects: Amino acids; Antifungal compounds; Biocontrol; Biomedical materials; Chromatography; Culture; Cyclic lipopeptides; Fatty acids; Homology; LC–MS; Microbial antagonism; Paenibacillus; Paenibacillus ehimensis; Purification; Residues; Strain; Paenibacillus ehimensis; Purification; Antifungal compounds; Microbial antagonism; Dab; Cyclic lipopeptides; LC–MS; PDA; Biocontrol
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2014.09.042

•Procedure of isolation of antifungal compounds from P. ehimensis is developed.•Antifungal compounds of P. ehimensis include two fractions of cyclic lipopeptides.•Main fraction contains bacillomycin L and fengycin/plipastatin/agrastatin families.•Other group comprises depsipeptides belonging to polypeptine family. Antifungal lipopeptides produced by an antagonistic bacterium, Paenibacillus ehimensis strain IB-X-b, were purified and analyzed. The acetone extract of the culture supernatant contained an antifungal amphiphilic fraction stainable with ninhydrin on thin layer chromatography. The fraction was further purified with water–methanol extraction followed by a chromatography on a C18-support. The analysis with LC–MS showed presence of two main series of homologous compounds, family of depsipeptides containing a hydroxy fatty acid, three 2,4-diaminobutyric acid (Dab) residues, five hydrophobic amino acids and one Ser/Thr residue, and cyclic lipopeptides of bacillomycin L and fengycin/plipastatin/agrastatin families. The prevailing compounds in this group are bacillomycin L-C15, fengycin/plipastatin A-C16 together with their homologues responsible for the majority of fungal growth inhibition by P. ehimensis IB-X-b.