Evaluation of a new real-time PCR assay (Check-Direct CPE) for rapid detection of KPC, OXA-48, VIM, and NDM carbapenemases using spiked rectal swabs

Abstract To prevent the spread of carbapenemase-producing bacteria, a fast and accurate detection of patients carrying these bacteria is extremely important. The Check-Direct CPE assay (Check-Points, Wageningen, The Netherlands) is a new multiplex real-time PCR assay, which has been developed to det...

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Published inDiagnostic microbiology and infectious disease Vol. 77; no. 4; pp. 316 - 320
Main Authors Nijhuis, Roel, Samuelsen, Ørjan, Savelkoul, Paul, van Zwet, Anton
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.12.2013
Subjects
Antibiotic
Bacterial Proteins - genetics
beta-Lactamases - genetics
Carbapenem resistance
Carriers
DNA, Bacterial
Enterobacteriaceae - classification
Enterobacteriaceae - genetics
Enterobacteriaceae - isolation & purification
Enterobacteriaceae Infections - diagnosis
Enterobacteriaceae Infections - microbiology
Humans
Infectious Disease
Internal Medicine
Multiplex Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Rectum - microbiology
Reproducibility of Results
Screening
Sensitivity and Specificity
Carriers
Screening
Antibiotic
Carbapenem resistance
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Summary:Abstract To prevent the spread of carbapenemase-producing bacteria, a fast and accurate detection of patients carrying these bacteria is extremely important. The Check-Direct CPE assay (Check-Points, Wageningen, The Netherlands) is a new multiplex real-time PCR assay, which has been developed to detect and differentiate between the most prevalent carbapenemase genes encountered in Enterobacteriaceae (blaKPC , blaOXA-48 , blaVIM , and blaNDM ) directly from rectal swabs. Evaluation of this assay using 83 non-duplicate isolates demonstrated 100% sensitivity and specificity and the correct identification of the carbapenemase gene(s) present in all carbapenemase-producing isolates. Moreover, the limit of detection (LoD) of the real-time PCR assay in spiked rectal swabs was determined and showed comparable LoDs with the ChromID CARBA agar. With an excellent performance on clinical isolates and spiked rectal swabs, this assay appeared to be an accurate and rapid method to detect blaKPC , blaOXA-48 , blaVIM , and blaNDM genes directly from a rectal screening swab.
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ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2013.09.007