Construct and face validity of a new model for the three-hit theory of depression using PACAP mutant mice on CD1 background

• Published in: Neuroscience Vol. 354; pp. 11 - 29
• Main Authors: Farkas, József, Kovács, László Á, Gáspár, László, Nafz, Anna, Gaszner, Tamás, Ujvári, Balázs, Kormos, Viktória, Csernus, Valér, Hashimoto, Hitoshi, Reglődi, Dóra, Gaszner, Balázs
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 23.06.2017
• Subjects: Adaptation, Ocular - physiology; Adrenal Glands - pathology; Animals; Animals, Newborn; Body Weight - genetics; chronic variable mild stress; Corticotropin-Releasing Hormone - blood; Corticotropin-Releasing Hormone - metabolism; CRF; Depression - blood; Depression - etiology; Depression - genetics; Depression - pathology; Disease Models, Animal; Exploratory Behavior - physiology; Female; FosB; Male; Maternal Deprivation; Mice; Mice, Knockout; Mutation - genetics; Neurology; Pituitary Adenylate Cyclase-Activating Polypeptide - genetics; Raphe Nuclei - pathology; Septal Nuclei - pathology; serotonin; Stress, Psychological - complications; Swimming - psychology; Urocortin1; BSTov; ACTH; PVN; centrally projecting Edinger–Westphal nucleus; phosphate-buffered saline; DR; knock out; serotonin; HZ; ethylene-diamine tetra-acetic acid; animal facility rearing; lightdark box; postnatal day; Ucn1; PBS; tail suspension test; specific signal density; PACAP; maternal deprivation; pituitary adenylate cyclase-activating polypeptide; FST; MD180; dorsal raphe nucleus; CORT; marble burying test; TST; CRF; HPA; wild type; maternal deprivation (180 min long); 5-HT; forced swim test; heterozygous; paraventricular nucleus; Urocortin1; CVMS; maternal separation (15 min long); central nucleus of amygdala; LDB; AFR; corticotropin-releasing factor; adrenocorticotropin; chronic variable mild stress; NDS; MANOVA; WT; MBT; hypothalamuspituitary–adrenal; KO; SSD; normal donkey serum; control; EDTA; multifactorial analysis of variance; MS15; cpEW; CTRL; PD; oval division of bed nucleus of stria terminalis; FosB; corticosterone; urocortin 1; CeA
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/j.neuroscience.2017.04.019

Highlights • A three-hit theory-based new animal model for depression was tested. • PACAP HZ mice with MD180 history upon CVMS showed depression-like phenotype. • The lack or lower expression of PACAP interacts with BSTov–CRF, cpEW–Ucn1, DR–5-HT. • Forced swim and marble burying tests supported the validity at behavioral level. • BSTov–CRF, cpEW–Ucn1, DR–5-HT neurons were affected in PACAP HZ mice.