Tea2p Kinesin Is Involved in Spatial Microtubule Organization by Transporting Tip1p on Microtubules

• Published in: Developmental cell Vol. 6; no. 6; pp. 831 - 843
• Main Authors: Busch, Karl Emanuel, Hayles, Jacky, Nurse, Paul, Brunner, Damian
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.06.2004
• Subjects: Carrier Proteins - genetics; Carrier Proteins - metabolism; Glycoproteins - genetics; Glycoproteins - metabolism; Heat-Shock Proteins; Intermediate Filament Proteins; Kinesin - genetics; Kinesin - metabolism; Microtubule-Associated Proteins - genetics; Microtubule-Associated Proteins - metabolism; Microtubules - genetics; Microtubules - metabolism; Neoplasm Proteins; Protein Transport - physiology; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Schizosaccharomyces - genetics; Schizosaccharomyces - growth & development; Schizosaccharomyces - metabolism; Schizosaccharomyces pombe; Schizosaccharomyces pombe Proteins - genetics; Schizosaccharomyces pombe Proteins - metabolism; Vesicular Transport Proteins
• ISSN: 1534-5807; 1878-1551
• DOI: 10.1016/j.devcel.2004.05.008

The positioning of growth sites in fission yeast cells is mediated by spatially controlled microtubule dynamics brought about by tip1p, a CLIP-170-like protein, which is localized at the microtubule tips and guides them to the cell ends. The kinesin tea2p is also located at microtubule tips and affects microtubule dynamics. Here we show that tea2p interacts with tip1p and that the two proteins move with high velocity along the microtubules toward their growing tips. There, tea2p and tip1p accumulate in larger particles. Particle formation requires the EB1 homolog, mal3p. Our results suggest a model in which kinesins regulate microtubule growth by transporting regulatory factors such as tip1p to the growing microtubule tips.