To Be, or Not to Be: That Is the Hamletic Question of Cryptic Evolution in the Eastern Atlantic and Mediterranean Raja miraletus Species Complex

• Published in: Animals (Basel) Vol. 13; no. 13; p. 2139
• Main Authors: Ferrari, Alice, Crobe, Valentina, Cannas, Rita, Leslie, Rob W, Serena, Fabrizio, Stagioni, Marco, Costa, Filipe O, Golani, Daniel, Hemida, Farid, Zaera-Perez, Diana, Sion, Letizia, Carbonara, Pierluigi, Fiorentino, Fabio, Tinti, Fausto, Cariani, Alessia
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.06.2023; MDPI
• Subjects: Bats; brown skate; cartilaginous fish; conservation biology; Cryptic species; Differentiation; Gene flow; Genetic diversity; Genetic structure; Genetic variability; Geographical distribution; Haplotypes; microsatellite loci; Mitochondrial DNA; Morphology; mtDNA; Oceans; Parasites; Phylogenetics; population genetics; Raja miraletus; Speciation; Species diversity; Taxa; Taxonomy; Angola; Indian Ocean; Senegal; Africa; South Africa; Israel; Mediterranean Sea; microsatellite loci; brown skate; population genetics; cartilaginous fish; conservation biology; mtDNA
• ISSN: 2076-2615; 2076-2615
• DOI: 10.3390/ani13132139

Despite a high species diversity, skates (Rajiformes) exhibit remarkably conservative morphology and ecology. Limited trait variations occur within and between species, and cryptic species have been reported among sister and non-sister taxa, suggesting that species complexes may be subject to stabilising selection. Three sibling species are currently recognised in the complex: (i) occurring along the Portuguese and Mediterranean coasts, (ii) in the Central-Eastern Atlantic off West Africa and (iii) in the Western Indian Ocean off South Africa. In the present study, the genetic variation at mitochondrial and nuclear markers was estimated in the species complex by analysing 323 individuals sampled across most of its geographical distribution area to test the hypothesis that restricted gene flow and genetic divergence within species reflect known climate and bio-oceanographic discontinuities. Our results support previous morphological studies and confirm the known taxonomic boundaries of the three recognised species. In addition, we identified multiple weakly differentiated clades in the Northeastern Atlantic Ocean and Mediterranean, at least two additional cryptic taxa off Senegal and Angola, a pronounced differentiation of ancient South African clades. The hidden genetic structure presented here may represent a valuable support to species' conservation action plans.