Evaluation of IP-10 in Quantiferon-Plus as biomarker for the diagnosis of latent tuberculosis infection

• Published in: Tuberculosis (Edinburgh, Scotland) Vol. 111; pp. 147 - 153
• Main Authors: Petrone, Linda, Vanini, Valentina, Chiacchio, Teresa, Petruccioli, Elisa, Cuzzi, Gilda, Schininà, Vincenzo, Palmieri, Fabrizio, Ippolito, Giuseppe, Goletti, Delia
• Format: Journal Article
• Language: English
• Published: Scotland Elsevier Ltd 01.07.2018; Elsevier Science Ltd
• Subjects: Adult; Bacterial Load; Biomarkers; Biomarkers - blood; Case-Control Studies; Chemokine CXCL10 - blood; Chemokine CXCL10 - immunology; Diagnosis; Enzyme-Linked Immunospot Assay - methods; Female; Host-Pathogen Interactions; Humans; Inflammation; Interferon; Interferon-gamma - blood; Interferon-gamma - immunology; Interferon-gamma Release Tests - methods; IP-10; Latent Tuberculosis - blood; Latent Tuberculosis - diagnosis; Latent Tuberculosis - immunology; Latent Tuberculosis - microbiology; LTBI; Male; Medical diagnosis; Middle Aged; Mycobacterium tuberculosis - immunology; Mycobacterium tuberculosis - pathogenicity; Peptides; Predictive Value of Tests; Quantiferon-plus; Reproducibility of Results; Sensitivity; Tuberculosis; Tubes; Quantiferon-plus; Inflammation; Tuberculosis; Diagnosis; IP-10; LTBI
• ISSN: 1472-9792; 1873-281X; 1873-281X
• DOI: 10.1016/j.tube.2018.06.005

The QuantiFERON-TB Gold Plus (QFT-Plus) is a new test for latent tuberculosis infection (LTBI) diagnosis, in which has been added a new tube containing shorter peptides stimulating CD8 T-cells and CD4-stimulating-peptides. Measurement of alternative biomarkers to Interferon-γ (IFN-γ) in QFT-Plus may improve its sensitivity. Interferon-γ inducible protein 10 (IP-10), has been proposed as a tuberculosis (TB) biomarker. We aimed to evaluate the IP-10 accuracy in QFT-Plus for LTBI diagnosis. QFT-Plus was performed in 36 active TB, 31 LTBI and 16 healthy donors (HD). IP-10 was detected by ELISA. IP-10 is increased in TB1 and TB2 tubes in subjects with active TB and LTBI compared to HD. A ROC analysis comparing active TB and HD was performed and a cut-off of 1174 pg/mL for TB1 and 928.8 pg/mL for TB2 identified active TB with 86% sensitivity (Se) and 94% specificity (Sp). Moreover, increased IP-10 in response to TB1 was found in subjects with LTBI compared to those with active TB. A cut-off point of ≥16,108 pg/mL was chosen to maximize the test performance. However, the test predicted LTBI only with 58% Se and 61% Sp. These results suggest that IP-10 is an alternative biomarker to IFN-γ in the QFT-Plus format.