Diazoxide and moderate-intensity exercise improve skeletal muscle function by decreasing oxidants and enhancing antioxidant defenses in hypertensive male rats

• Published in: Physiological reports Vol. 12; no. 8; p. e16026
• Main Authors: Bravo Sánchez, Estefanía, Nolasco Ruíz, César J, Gómez-Barroso, Mariana, Cortés Rojo, Christian, Rodríguez Orozco, Alain R, Saavedra Molina, Alfredo, Manzo Ávalos, Salvador, Montoya Pérez, Rocío
• Format: Journal Article
• Language: English
• Published: United States John Wiley & Sons, Inc 01.04.2024; John Wiley and Sons Inc; Wiley
• Subjects: Animals; Antihypertensives; antioxidant; Antioxidants; Antioxidants - metabolism; Antioxidants - pharmacology; Biomarkers; Blood pressure; diazoxide; Diazoxide - pharmacology; Diet; exercise; Fluorescent indicators; Glucose; Hypertension; Hypertension - metabolism; Hypertension - physiopathology; Insulin; Insulin secretion; Laboratory animals; Male; Metabolism; Muscle contraction; Muscle function; Muscle, Skeletal - drug effects; Muscle, Skeletal - metabolism; Musculoskeletal system; Original; Oxidants; Oxidants - metabolism; Oxidative Stress - drug effects; Physical Conditioning, Animal - physiology; Potassium; Protocol; Rats; Rats, Wistar; Rodents; Skeletal muscle; Sodium; Sodium chloride; Soleus muscle; United States > US; Sarasota Florida; exercise; antioxidant; skeletal muscle; diazoxide; hypertension
• ISSN: 2051-817X
• DOI: 10.14814/phy2.16026

High sodium intake is decisive in the incidence increase and prevalence of hypertension, which has an impact on skeletal muscle functionality. Diazoxide is an antihypertensive agent that inhibits insulin secretion and is an opener of K channels (adosine triphosphate sensitive potasium channels). For this reason, it is hypothesized that moderate-intensity exercise and diazoxide improve skeletal muscle function by reducing the oxidants in hypertensive rats. Male Wistar rats were assigned into eight groups: control (CTRL), diazoxide (DZX), exercise (EX), exercise + diazoxide (EX + DZX), hypertension (HTN), hypertension + diazoxide (HTN + DZX), hypertension + exercise (HTN + EX), and hypertension + exercise + diazoxide (HTN + EX + DZX). To induce hypertension, the rats received 8% NaCl dissolved in water orally for 30 days; in the following 8 weeks, 4% NaCl was supplied to maintain the pathology. The treatment with physical exercise of moderate intensity lasted 8 weeks. The administration dose of diazoxide was 35 mg/kg intraperitoneally for 14 days. Tension recording was performed on the extensor digitorum longus and the soleus muscle. Muscle homogenates were used to measure oxidants using fluorescent probe and the activity of antioxidant systems. Diazoxide and moderate-intensity exercise reduced oxidants and increased antioxidant defenses.