Enzymatic hydrolysis and characterization of waste lignocellulosic biomass produced after dye bioremediation under solid state fermentation

• Published in: Bioresource technology Vol. 168; pp. 136 - 141
• Main Authors: Waghmare, Pankajkumar R., Kadam, Avinash A., Saratale, Ganesh D., Govindwar, Sanjay P.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Kidlington Elsevier Ltd 01.09.2014; Elsevier
• Subjects: Adsorption; Applied sciences; biodegradation; Biodegradation, Environmental; biofuels; Biological and medical sciences; Biomass; Bioremediation; Biotechnology; Cellulase - metabolism; Cellulose - ultrastructure; Chromatography, Thin Layer; Coloring Agents - isolation & purification; Crystallization; Environment and pollution; Enzymatic hydrolysis; Exact sciences and technology; Fermentation; Fourier transform infrared spectroscopy; fuel production; Fundamental and applied biological sciences. Psychology; Hydrolysis; Industrial applications and implications. Economical aspects; Lignin - metabolism; lignocellulose; Lignocellulosic biomass; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Oxidation-Reduction; pentoses; Phanerochaete - enzymology; Phanerochaete chrysosporium; Pollution; reducing sugars; saccharification; Saccharum - chemistry; scanning electron microscopy; Solid state fermentation; Spectroscopy, Fourier Transform Infrared; sugarcane bagasse; Surface Properties; Textiles; Waste Products; Wastes; X-Ray Diffraction; Bioremediation; Lignocellulosic biomass; Enzymatic hydrolysis; Phanerochaete chrysosporium; Solid state fermentation; Dyes; Pollution control; Lignocellulosics; Biomass; Basidiomycota; Fungi; Hydrolysis; Wood destroying fungi; Enzymatic digestion; Solid substrate fermentation; Wastes
• ISSN: 0960-8524; 1873-2976; 1873-2976
• DOI: 10.1016/j.biortech.2014.02.099

[Display omitted] •Adsoption of textile dye RB172 on agricultural waste SCB biomass.•Biodegradation of SCB adsorbed RB172 under solid state fermentation by P. stuarti.•Production of cellulolytic crude enzyme from Phanerochaete chrysosporium NCIM 1106.•Enzymatic hydrolysis of waste biomass (ddSCB) obtained after bioremediation of RB172.•HPTLC, FTIR, XRD and SEM characterization of SCB and ddSCB after enzymatic hydrolysis. Sugarcane bagasse (SCB) adsorbes 60% Reactive Blue172 (RB172). Providensia staurti EbtSPG able to decolorize SCB adsorbed RB172 up to 99% under solid state fermentation (SSF). The enzymatic saccharification efficiency of waste biomass after bioremediation of RB172 process (ddSCB) has been evaluated. The cellulolyitc crude enzyme produced by Phanerochaete chrysosporium used for enzymatic hydrolysis of native SCB and ddSCB which produces 0.08 and 0.3g/L of reducing sugars respectively after 48h of incubation. The production of hexose and pentose sugars during hydrolysis was confirmed by HPTLC. The effect of enzymatic hydrolysis on SCB and ddSCB has been evaluated by FTIR, XRD and SEM analysis. Thus, during dye biodegradation under SSF causes biological pretreatment of SCB which significantly enhanced its enzymatic saccharification. Adsorption of dye on SCB, its bioremediation under SSF produces wastes biomass and which further utilized for enzymatic saccharification for biofuel production.