Long Non-Coding RNA BANCR Promotes Endometrial Cancer Cell Proliferation and Invasion by Regulating MMP2 and MMP1 via ERK/MAPK Signaling Pathway

• Published in: Cellular physiology and biochemistry Vol. 40; no. 3-4; pp. 644 - 656
• Main Authors: Wang, Danni, Wang, Danbo, Wang, Ning, Long, Zaiqiu, Ren, Xuemei
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland Cell Physiol Biochem Press GmbH & Co KG 01.01.2016
• Subjects: BANCR; Blotting, Western; Cell Cycle - genetics; Cell Line, Tumor; Cell Movement - genetics; Cyclin D1 - metabolism; Endometrial Neoplasms - enzymology; Endometrial Neoplasms - genetics; Endometrial Neoplasms - pathology; ERK/MAPK; Female; Gene Expression Regulation, Neoplastic; Humans; LncRNA; MAP Kinase Signaling System; Matrix Metalloproteinase 1 - genetics; Matrix Metalloproteinase 1 - metabolism; Matrix Metalloproteinase 2 - genetics; Matrix Metalloproteinase 2 - metabolism; Middle Aged; MMP1; MMP2; Neoplasm Invasiveness; Original Paper; Proto-Oncogene Proteins c-bcl-2 - metabolism; RNA, Long Noncoding - genetics; RNA, Long Noncoding - metabolism; RNA, Small Interfering - metabolism; Transfection; Type 1 endometrial cancer; LncRNA; Type 1 endometrial cancer; ERK/MAPK; MMP1; MMP2; BANCR
• ISSN: 1015-8987; 1421-9778
• DOI: 10.1159/000452577

Background/Aims: Microarray screening had found BRAF-activated non-coding RNA (BANCR) was significantly upregulated in type 1 endometrial cancer (EC). This study aimed to assess the potential role of long non-coding RNA (lncRNA) BANCR in the pathogenesis and progression of type 1 EC. Methods: Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to confirm the expression of BANCR in type 1 EC tissue, and analyze its clinical significance. In vitro, RNA interference (siRNA) was used to investigate the biological role of BANCR in type 1 EC. Results: qRT-PCR revealed that the expression of lncRNA BANCR was higher in type 1 EC (P<0.01). BANCR expression was significantly correlated with FIGO stage, pathological grade, myometrial invasion, and lymph node metastasis. The expression of BANCR was significantly correlated with that of MMP2/MMP1. In vitro, knockdown of BANCR significantly suppressed proliferation, migration, and invasion of Ishikawa and HEC-1A cells, and significantly inhibited the ERK/MAPK signaling pathway that decreased MMP2 and MMP1 expression. Conclusion: BANCR is highly expressed in type 1 EC tissue and promotes EC-cell proliferation, migration, and invasion by activating ERK/MAPK signaling pathway that regulates MMP2/MMP1 expression. BANCR is expected to become a prognostic marker and therapeutic target in type 1 EC.