Kinetic Schemes for Post-Synchronized Single Molecule Dynamics

Recordings from single molecule experiments can be aggregated to determine average kinetic properties of the system under observation. The kinetics after a synchronized reaction step can be interpreted using all of the standard tools developed for ensemble perturbation experiments. The kinetics lead...

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Bibliographic Details
Published inBiophysical journal Vol. 102; no. 6; pp. L23 - L25
Main Authors Chen, Chunlai, Greenberg, Michael J., Laakso, Joseph M., Ostap, E. Michael, Goldman, Yale E., Shuman, Henry
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 21.03.2012
Biophysical Society
The Biophysical Society
Subjects
Actomyosin - metabolism
Biophysical Letter
Biophysics
Fluorescence Resonance Energy Transfer
Kinetics
molecular dynamics
Molecular Dynamics Simulation
Molecules
Optical Tweezers
Peptide Elongation Factor G - metabolism
Protein Biosynthesis
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Summary:Recordings from single molecule experiments can be aggregated to determine average kinetic properties of the system under observation. The kinetics after a synchronized reaction step can be interpreted using all of the standard tools developed for ensemble perturbation experiments. The kinetics leading up to a synchronized event, determined by the lifetimes of the preceding states; however, are not as obvious if the reaction has reversible steps or branches. Here we describe a general procedure for dealing with these situations.
Bibliography:http://dx.doi.org/10.1016/j.bpj.2012.01.054
ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
ISSN:0006-3495
1542-0086
DOI:10.1016/j.bpj.2012.01.054