Cyclooxygenase-2 polymorphisms and the risk of esophageal adeno- or squamous cell carcinoma

• Published in: World journal of gastroenterology : WJG Vol. 15; no. 28; pp. 3493 - 3497
• Main Authors: Kristinsson, Jón O, van Westerveld, Paul, te Morsche, Rene H M, Roelofs, Hennie M J, Wobbes, T, Witteman, Ben J M, Tan, Adriaan C I T L, van Oijen, Martijn G H, Jansen, Jan B M J, Peters, Wilbert H M
• Format: Journal Article
• Language: English
• Published: United States Department of Gastroenterology, Radboud University Nijmegen Medical Center, PO Box 9101, 6500 HB Nijmegen, The Netherlands%Department of Surgery, Radboud University Nijmegen Medical Center, PO Box 9101, 6500 HB Nijmegen, The Netherlands%Department of Gastroenterology, Hospital Gelderse Vallei, PO Box 9025, 6710 HN, Ede, The Netherlands%Adriaan CITL Tan, Department of Gastroenterology, Canisius Wilhelmina Hospital, Weg door Jonkerbos 100, 6532 SZ, Nijmegen, The Netherlands 28.07.2009; The WJG Press
• Subjects: Adenocarcinoma - genetics; Aged; Brief; Carcinoma, Squamous Cell - genetics; Cyclooxygenase 2 - genetics; Esophageal Neoplasms - genetics; Female; Genetic Predisposition to Disease; Humans; Male; Middle Aged; Odds Ratio; Polymorphism, Single Nucleotide; 基因多态性; 环氧合酶-2; 琼脂糖凝胶电泳; 遗传算法; 限制性内切酶; 食管癌; Adenocarcinoma; Squamous cell carcinoma; Cyclooxygenase-2; Genetic polymorphism; Esophagus
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.15.3493

AIM： TO determine whether -1195 A→G and/or -765 G→C polymorphisms in Cyclooxygenase-2 CCOX-2） may have a risk modifying effect on the development of esophageal carcinoma in a Dutch Caucasian population. METHODS： Two study groups were recruited, 252 patients with esophageal carcinoma and 240 healthy controls, matched for race, age, gender and recruiting area. DNA was isolated from whole blood and used for genotyping. PCR products were digested with restriction enzymes and products were analyzed by agarose gel electrophoresis. Odds ratios （OR） and 95% confidence intervals （CI） were estimated. RESULTS： The distribution of the -1195A→G polymorphism was significantly different in esophageal cancer patients compared to controls. The -1195 GG genotype resulted in a higher risk of developing esophageal adenocarcinoma （OR = 3.85, 95% CI： 1.45-10.3） compared with the -1195AA genotype as a reference. The -765 G→C genotype distribution was not different between the two groups. The GG/ GG haplotype was present more often in esophageal adenocarcinoma patients than in controls （OR = 3.45, 95% CI： 1.24-9.58; with AG/AG as a reference）. The same trends were observed in patients with squamous cell carcinomas, however, the results did not reach statistical significance. CONCLUSION： Presence of the COX-2 -1195 GG genotype and of the GG/GG haplotype may result in a higher risk of developing esophageal carcinoma.