Bioelectronic cell-based device provides a strategy for the treatment of the experimental model of multiple sclerosis

• Published in: Journal of controlled release Vol. 352; pp. 994 - 1008
• Main Authors: Audouard, Emilie, Michel, Fanny, Pierroz, Vanessa, Kim, Taeuk, Rousselot, Lisa, Gillet-Legrand, Béatrix, Dufayet-Chauffaut, Gaëlle, Buchmann, Peter, Florea, Michael, Khel, Alexander, Altynbekova, Kamilya, Delgaldo, Claudia, Escudero, Encarna, Soler, Alejandra Ben Aissa, Cartier, Nathalie, Piguet, Francoise, Folcher, Marc
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2022; Elsevier; Elsevier Science Publishers
• Subjects: Animals; Bioelectronic; Cell-based device; Disease Models, Animal; Drug delivery; Encephalomyelitis, Autoimmune, Experimental - therapy; Gene Expression; Humans; Interferon; Interferon-beta - genetics; Interferon-beta - metabolism; Life Sciences; Mice; Mice, Inbred C57BL; Multiple sclerosis; Multiple Sclerosis - therapy; Neurons and Cognition; Optogenetic; Multiple sclerosis; Drug delivery; Interferon; Cell-based device; Bioelectronic; Optogenetic
• ISSN: 0168-3659; 1873-4995
• DOI: 10.1016/j.jconrel.2022.11.008

Wireless powered optogenetic cell-based implant provides a strategy to deliver subcutaneously therapeutic proteins. Immortalize Human Mesenchymal Stem Cells (hMSC-TERT) expressing the bacteriophytochrome diguanylate cyclase (DGCL) were validated for optogenetic controlled interferon-β delivery (Optoferon cells) in a bioelectronic cell-based implant. Optoferon cells transcriptomic profiling was used to elaborate an in-silico model of the recombinant interferon-β production. Wireless optoelectronic device integration was developed using additive manufacturing and injection molding. Implant cell-based optoelectronic interface manufacturing was established to integrate industrial flexible compact low-resistance screen-printed Near Field Communication (NFC) coil antenna. Optogenetic cell-based implant biocompatibility, and device performances were evaluated in the Experimental Autoimmune Encephalomyelitis (EAE) mouse model of multiple sclerosis. [Display omitted] •Synthetic optogenetic pathway can be engineered in multiple cell lineage.•Bioelectronic device allows optogenetic controlled secretion of therapeutic proteins.•Screen printed near field communication (NFC) antenna supports implantable bioelectronic.•Industrial manufacturing process for bioelectronic cell-based device is established.•Interferon beta disease modifying therapy can be administered with a bioelectronic implant.