Extraction of extracellular lipids from chemoautotrophic bacteria Serratia sp. ISTD04 for production of biodiesel

• Published in: Bioresource technology Vol. 165; pp. 201 - 204
• Main Authors: Bharti, Randhir K., Srivastava, Shaili, Thakur, Indu Shekhar
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Kidlington Elsevier Ltd 01.08.2014; Elsevier
• Subjects: Bacteria; Biodiesel; Biofuel production; Biofuels; Biological and medical sciences; Biotechnology; Biotechnology - methods; Carbon dioxide; Carbon Dioxide - metabolism; Chemoautotrophic Growth; Energy; Esters; Esters - metabolism; Extracellular lipids; Extracellular Space - chemistry; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; Lipids; Lipids - isolation & purification; Oleic acid; Serratia; Serratia - metabolism; Strain; Transesterification processes; Transesterification processes; Biodiesel; Extracellular lipids; Bacteria; Lipids; Transesterification; Extracellular; Serratia; Enterobacteriaceae
• ISSN: 0960-8524; 1873-2976
• DOI: 10.1016/j.biortech.2014.02.075

•Serratia sp. ISTD04 isolated from marble rock for CO2 sequestration.•Extraction of extracellular lipids from bacteria.•Transesterification indicated formation of biodiesel from lipids.•Saturated and unsaturated FAME was found to be 51% and 49% respectively. A CO2 sequestering bacterial strain, Serratia sp. ISTD04, that produces a significant amount of extracellular lipids was isolated from marble mine rocks. 14C labeling analysis revealed that the rate of assimilation of CO2 by the strain is 0.756×10−9μmolCO2fixedcell−1h−1. It was found to produce 466mg/l of extracellular lipid which was characterized using 1H NMR. After transesterification of lipids, the total saturated and unsaturated FAME was found to be 51% and 49% respectively. The major FAME contained in the biodiesel were palmitic acid methyl ester (C16:0), oleic acid methyl ester (C18:1) and 10-nonadecenoic acid methyl ester (C19:1). Biodiesel produced by Serratia sp. ISTD04 is balanced in terms of FAME composition of good quality. It also contained higher proportion of oleic acid (35%) which makes it suitable for utilization in existing engines. Thus, the strain can be harnessed commercially to sequester CO2 into biodiesel.