Apoptosis of human pancreatic cancer cells induced by Triptolide
AIM: To investigate apoptosis in human pancreatic cancer cells induced by Triptolide (TL), and the relationship between this apoptosis and expression of caspase-3' bcl-2 and bax. METHODS: Human pancreatic cancer cell line SW1990 was cultured in DMEM media for this study. MTT assay was used to determ...
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Published in | World journal of gastroenterology : WJG Vol. 14; no. 10; pp. 1504 - 1509 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Department of Gastroenterology,Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China
14.03.2008
The WJG Press and Baishideng |
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Abstract | AIM: To investigate apoptosis in human pancreatic cancer cells induced by Triptolide (TL), and the relationship between this apoptosis and expression of caspase-3' bcl-2 and bax. METHODS: Human pancreatic cancer cell line SW1990 was cultured in DMEM media for this study. MTT assay was used to determine the cell growth inhibitory rate in vitro. Flow cytometry and TUNEL assay were used to detect the apoptosis of human pancreatic cancer cells before and after TL treatment. RT-PCR was used to detect the expression of apoptosis-associated gene caspase-3' bcl-2 and bax. RESULTS: TL inhibited the growth of human pancreatic cancer cells in a dose-and time-dependent manner. TL induced human pancreatic cancer cells to undergo apoptosis with typically apoptotic characteristics. TUNEL assay showed that after the treatment of human pancreatic cancer cells with 40 ng/mL TL for 12 h and 24 h, the apoptotic rates of human pancreatic cancer cells increased significantly. RT-PCR demonstrated that caspase-3 and bax were significantly up-regulated in SW1990 cells treated with TL while bcl-2 mRNA was not. CONCLUSION: TL is able to induce the apoptosis in human pancreatic cancer cells. This apoptosis may be mediated by up-regulating the expression of apoptosisassociated caspase-3 and bax gene. |
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AbstractList | AIM: To investigate apoptosis in human pancreatic cancer cells induced by Triptolide (TL), and the relationship between this apoptosis and expression of caspase-3’ bcl-2 and bax.
METHODS: Human pancreatic cancer cell line SW1990 was cultured in DMEM media for this study. MTT assay was used to determine the cell growth inhibitory rate
in vitro
. Flow cytometry and TUNEL assay were used to detect the apoptosis of human pancreatic cancer cells before and after TL treatment. RT-PCR was used to detect the expression of apoptosis-associated gene caspase-3’ bcl-2 and bax.
RESULTS: TL inhibited the growth of human pancreatic cancer cells in a dose-and time-dependent manner. TL induced human pancreatic cancer cells to undergo apoptosis with typically apoptotic characteristics. TUNEL assay showed that after the treatment of human pancreatic cancer cells with 40 ng/mL TL for 12 h and 24 h, the apoptotic rates of human pancreatic cancer cells increased significantly. RT-PCR demonstrated that caspase-3 and bax were significantly up-regulated in SW1990 cells treated with TL while bcl-2 mRNA was not.
CONCLUSION: TL is able to induce the apoptosis in human pancreatic cancer cells. This apoptosis may be mediated by up-regulating the expression of apoptosis-associated caspase-3 and bax gene. To investigate apoptosis in human pancreatic cancer cells induced by Triptolide (TL), and the relationship between this apoptosis and expression of caspase-3' bcl-2 and bax. Human pancreatic cancer cell line SW1990 was cultured in DMEM media for this study. MTT assay was used to determine the cell growth inhibitory rate in vitro. Flow cytometry and TUNEL assay were used to detect the apoptosis of human pancreatic cancer cells before and after TL treatment. RT-PCR was used to detect the expression of apoptosis-associated gene caspase-3' bcl-2 and bax. TL inhibited the growth of human pancreatic cancer cells in a dose-and time-dependent manner. TL induced human pancreatic cancer cells to undergo apoptosis with typically apoptotic characteristics. TUNEL assay showed that after the treatment of human pancreatic cancer cells with 40 ng/mL TL for 12 h and 24 h, the apoptotic rates of human pancreatic cancer cells increased significantly. RT-PCR demonstrated that caspase-3 and bax were significantly up-regulated in SW1990 cells treated with TL while bcl-2 mRNA was not. TL is able to induce the apoptosis in human pancreatic cancer cells. This apoptosis may be mediated by up-regulating the expression of apoptosis-associated caspase-3 and bax gene. AIM: To investigate apoptosis in human pancreatic cancer cells induced by Triptolide (TL), and the relationship between this apoptosis and expression of caspase-3' bcl-2 and bax. METHODS: Human pancreatic cancer cell line SW1990 was cultured in DMEM media for this study. MTT assay was used to determine the cell growth inhibitory rate in vitro. Flow cytometry and TUNEL assay were used to detect the apoptosis of human pancreatic cancer cells before and after TL treatment. RT-PCR was used to detect the expression of apoptosis-associated gene caspase-3' bcl-2 and bax. RESULTS: TL inhibited the growth of human pancreatic cancer cells in a dose-and time-dependent manner. TL induced human pancreatic cancer cells to undergo apoptosis with typically apoptotic characteristics. TUNEL assay showed that after the treatment of human pancreatic cancer cells with 40 ng/mL TL for 12 h and 24 h, the apoptotic rates of human pancreatic cancer cells increased significantly. RT-PCR demonstrated that caspase-3 and bax were significantly up-regulated in SW1990 cells treated with TL while bcl-2 mRNA was not. CONCLUSION: TL is able to induce the apoptosis in human pancreatic cancer cells. This apoptosis may be mediated by up-regulating the expression of apoptosisassociated caspase-3 and bax gene. R73; AIM:To investigate apoptosis in human pancreatic cancer ceils induced by Triptolide (TL),and the relationship between this apoptosis and expression of caspase-3' bcl-2 and bax.METHODS:Human pancreatic cancer cell line SW1990 was cultured in DIEM media for this study.MTT assay was used to determine the cell growth inhibitory rate in vitro.Flow cytometry and TUNEL assay were used to detect the apoptosis of human pancreatic cancer cells before and after TL treatment.RT-PCR was used to detect the expression of apoptosis-associated gene caspase-3' bcl-2 and bax.RESULTS:TL inhibited the growth of human pancreatic cancer cells in a dose-and time-dependent manner.TL induced human pancreatic cancer cells to undergo apoptosis with typically apoptotic characteristics.TUNEL assay showed that after the treatment of human pancreatic cancer cells with 40 ng/mL TL for 12 h and 24 h,the apoptotic rates of human pancreatic cancer cells increased significantly.RT-PCR demonstrated that caspase-3 and bax were significantly up-regulated in SW1990 cells treated with TL while bcl-2 mRNA was not.CONCLUSION:TL is able to induce the apoptosis in human pancreatic cancer cells.This apoptosis may be mediated by up-regulating the expression of apoptosisassociated caspase-3 and bax gene. |
Author | Guo-Xiong Zhou Xiao-Ling Ding Jie-Fei Huang Hong Zhang Sheng-Bao Wu Jian-Ping Cheng Qun Wei |
AuthorAffiliation | Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China |
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Cites_doi | 10.3322/canjclin.50.1.7 10.1038/emm.2002.64 10.1016/j.phytochem.2007.02.021 10.1016/S0378-4274(01)00353-8 10.1021/ja00775a078 10.3322/canjclin.46.1.5 10.1182/blood-2005-09-3898 10.1016/S0162-3109(98)00036-8 10.1016/j.bbagen.2007.03.002 10.1016/S0304-3835(96)04554-5 10.1038/sj.onc.1204981 10.1158/0008-5472.CAN-07-1077 10.1074/jbc.M009713200 10.1007/s00109-005-0022-4 10.1074/jbc.274.19.13443 10.1074/jbc.274.19.13451 10.1007/s002689900598 10.1177/147323000703500508 10.1016/j.leukres.2004.05.014 10.1016/j.bbrc.2004.04.201 10.1016/S0147-0272(96)80001-8 10.3109/10428190109064582 |
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Keywords | Bcl-2 Bax Caspase-3 Triptolide Pancreatic cancer Apoptosis |
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Notes | Bcl-2 Triptolide; Pancreatic cancer; Apoptosis;Bcl-2; Caspase-3; Bax 14-1219/R Triptolide Pancreatic cancer Bax Caspase-3 R735.9 Apoptosis Telephone: +86-513-85052079 Author contributions: Zhou GX, Ding XL contributed equally to this work; Zhou GX, Ding XL, Huang JF, Zhang H and Wu SB designed research; Ding XL, Zhang H, Wu SB, Cheng JP and Wei Q performed research; Zhou GX, Ding XL and Zhang H analyzed data; Zhou GX and Ding XL wrote the paper. Fax: +86-513-85519820 Correspondence to: Guo-Xiong Zhou, Department of Gastro-enterology, Affiliated Hospital of Nantong University, Nantong 226001, Jiangsu Province, China. zhouguoxiong@medmail.com.cn |
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Snippet | AIM: To investigate apoptosis in human pancreatic cancer cells induced by Triptolide (TL), and the relationship between this apoptosis and expression of... To investigate apoptosis in human pancreatic cancer cells induced by Triptolide (TL), and the relationship between this apoptosis and expression of caspase-3'... R73; AIM:To investigate apoptosis in human pancreatic cancer ceils induced by Triptolide (TL),and the relationship between this apoptosis and expression of... AIM: To investigate apoptosis in human pancreatic cancer cells induced by Triptolide (TL), and the relationship between this apoptosis and expression of... |
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SubjectTerms | Adenocarcinoma - metabolism Adenocarcinoma - pathology Antineoplastic Agents, Alkylating - pharmacology Apoptosis - drug effects Basic Research bcl-2-Associated X Protein - metabolism Caspase 3 - metabolism Cell Line, Tumor Cell Proliferation - drug effects Diterpenes - pharmacology Dose-Response Relationship, Drug Drugs, Chinese Herbal - pharmacology Epoxy Compounds - pharmacology Gene Expression Regulation, Neoplastic - drug effects Humans Pancreatic Neoplasms - metabolism Pancreatic Neoplasms - pathology Phenanthrenes - pharmacology Proto-Oncogene Proteins c-bcl-2 - metabolism RNA, Messenger - metabolism Time Factors Tripterygium Up-Regulation - drug effects 癌细胞 细胞凋亡 胰腺癌 雷公藤甲素 |
Title | Apoptosis of human pancreatic cancer cells induced by Triptolide |
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