Apoptosis of human pancreatic cancer cells induced by Triptolide

• Published in: World journal of gastroenterology : WJG Vol. 14; no. 10; pp. 1504 - 1509
• Main Authors: Zhou, Guo-Xiong, Ding, Xiao-Ling, Huang, Jie-Fei, Zhang, Hong, Wu, Sheng-Bao, Cheng, Jian-Ping, Wei, Qun
• Format: Journal Article
• Language: English
• Published: United States Department of Gastroenterology,Affiliated Hospital of Nantong University,Nantong 226001,Jiangsu Province,China 14.03.2008; The WJG Press and Baishideng
• Subjects: Adenocarcinoma - metabolism; Adenocarcinoma - pathology; Antineoplastic Agents, Alkylating - pharmacology; Apoptosis - drug effects; Basic Research; bcl-2-Associated X Protein - metabolism; Caspase 3 - metabolism; Cell Line, Tumor; Cell Proliferation - drug effects; Diterpenes - pharmacology; Dose-Response Relationship, Drug; Drugs, Chinese Herbal - pharmacology; Epoxy Compounds - pharmacology; Gene Expression Regulation, Neoplastic - drug effects; Humans; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Phenanthrenes - pharmacology; Proto-Oncogene Proteins c-bcl-2 - metabolism; RNA, Messenger - metabolism; Time Factors; Tripterygium; Up-Regulation - drug effects; 癌细胞; 细胞凋亡; 胰腺癌; 雷公藤甲素; Bcl-2; Bax; Caspase-3; Triptolide; Pancreatic cancer; Apoptosis
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.14.1504

AIM： To investigate apoptosis in human pancreatic cancer cells induced by Triptolide （TL）, and the relationship between this apoptosis and expression of caspase-3＇ bcl-2 and bax. METHODS： Human pancreatic cancer cell line SW1990 was cultured in DMEM media for this study. MTT assay was used to determine the cell growth inhibitory rate in vitro. Flow cytometry and TUNEL assay were used to detect the apoptosis of human pancreatic cancer cells before and after TL treatment. RT-PCR was used to detect the expression of apoptosis-associated gene caspase-3＇ bcl-2 and bax. RESULTS： TL inhibited the growth of human pancreatic cancer cells in a dose-and time-dependent manner. TL induced human pancreatic cancer cells to undergo apoptosis with typically apoptotic characteristics. TUNEL assay showed that after the treatment of human pancreatic cancer cells with 40 ng/mL TL for 12 h and 24 h, the apoptotic rates of human pancreatic cancer cells increased significantly. RT-PCR demonstrated that caspase-3 and bax were significantly up-regulated in SW1990 cells treated with TL while bcl-2 mRNA was not. CONCLUSION： TL is able to induce the apoptosis in human pancreatic cancer cells. This apoptosis may be mediated by up-regulating the expression of apoptosisassociated caspase-3 and bax gene.