Highly sensitive and robust peroxidase-like activity of porous nanorods of ceria and their application for breast cancer detection

Abstract Porous nanorods of ceria ( PN -Ceria), a novel ceria nanostructure with a large surface area and a high surface Ce3+ fraction, exhibited strong intrinsic peroxidase activity toward a classical peroxidase substrate in the presence of H2 O2 . Peroxidase-like activity of ceria originated from...

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Published inBiomaterials Vol. 59; pp. 116 - 124
Main Authors Tian, Zhimin, Li, Jing, Zhang, Zhiyun, Gao, Wei, Zhou, Xuemei, Qu, Yongquan
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier Ltd 01.08.2015
Subjects
Advanced Basic Science
Biomimetic
Breast
Breast cancer detection
Breast Neoplasms - diagnosis
Cancer
Catalysis
Catalysts
Cerium - chemistry
Cerium oxide
Dentistry
ELISA
Enzymes
Female
Humans
Kinetics
Microscopy, Electron, Transmission
Nanorods
Nanotubes
Peroxidase
Peroxidase mimetics
Peroxidases - metabolism
Porosity
Porous nanorods of ceria
Breast cancer detection
Biomimetic
Porous nanorods of ceria
Peroxidase mimetics
ELISA
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Summary:Abstract Porous nanorods of ceria ( PN -Ceria), a novel ceria nanostructure with a large surface area and a high surface Ce3+ fraction, exhibited strong intrinsic peroxidase activity toward a classical peroxidase substrate in the presence of H2 O2 . Peroxidase-like activity of ceria originated from surface Ce3+ species as the catalytic center, thereby explaining the high performance of PN -Ceria as an artificial enzyme mimicking peroxidase. Compared with the natural enzyme horseradish peroxidase (HRP), PN -Ceria showed several advantages such as low cost, easy storage, high sensitivity, and, prominently, chemical and catalytic stability under harsh conditions. Importantly, the enzymatic activity of PN -Ceria remained nearly constant and stable over a wide range of temperature and pH values, ensuring the accuracy and reliability of measurements of its peroxidase-like activity. A PN -Ceria based novel diagnostic system was developed for breast cancer detection with a higher sensitivity than the standard HRP detection system. Our work has laid a solid foundation for the development of PN -Ceria as a novel diagnostic tool for clinical use.
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ISSN:0142-9612
1878-5905
DOI:10.1016/j.biomaterials.2015.04.039